Document Detail


Impact of PAMAM G2 and G6 dendrimers on bovine serum albumin (fatty acids free and loaded with different fatty acids).
MedLine Citation:
PMID:  18068340     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dendrimers are a new class of nanotechnological polymers suitable for drug targeting, microarray systems or detoxication. The present study is devoted to a detailed analysis of binding between PAMAM dendrimers and bovine serum albumin (fatty acid free or loaded with oleic, linoleic, oleic+linoleic or oleic+linoleic+arachodonic acids) by measuring zeta-potential, fluorescence quenching, fluorescence anisotropy and electron paramagnetic resonance. Addition of PAMAM G2 and G6 dendrimers to protein solutions resulted in attachment to the protein molecule. The PAMAM dendrimers also competed with BSA for fatty acids if two or three fatty acids were loaded per protein. This can lead to the extraction of fatty acids from BSA to the PAMAM dendrimer.
Authors:
Dzmitry Shcharbin; M Francesca Ottaviani; Michela Cangiotti; Magdalena Przybyszewska; Marian Zaborski; Maria Bryszewska
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-04
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  63     ISSN:  0927-7765     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-03-24     Completed Date:  2008-06-23     Revised Date:  2009-10-16    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Affiliation:
Department of General Biophysics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. shcharbi@biol.uni.lodz.pl <shcharbi@biol.uni.lodz.pl>
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MeSH Terms
Descriptor/Qualifier:
Anisotropy
Dendrimers / chemistry*
Electrochemistry
Electron Spin Resonance Spectroscopy
Fatty Acids / chemistry*
Polyamines / chemistry*
Protein Binding
Serum Albumin, Bovine / chemistry*
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Dendrimers; 0/Fatty Acids; 0/PAMAM Starburst; 0/Polyamines; 0/Serum Albumin, Bovine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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