Document Detail


Impact of the NK cell receptor LIR-1 (ILT-2/CD85j/LILRB1) on cytotoxicity against multiple myeloma.
MedLine Citation:
PMID:  22844324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of different receptors in natural-killer- (NK-) cell-mediated cytotoxicity against multiple myeloma (MM) cells is unknown. We investigated if an enhancement of NK-cell-mediated cytotoxicity against MM could be reached by blocking of the inhibitory leukocyte immunoglobulin-like receptor 1 (LIR-1). Our investigations revealed high levels of LIR-1 expression not only on the NK cell line NK-92, but also on myeloma cells (MOLP-8, RPMI8226) as well as on a lymphoblastoid cell line (LBCL; IM-9). Subsequent cytotoxicity assays were designed to show the isolated effects of LIR-1 blocking on either the effector or the tumor side to rule out receptor-receptor interactions. Although NK-92 was shown to be capable of myeloma cell lysis, inhibition of LIR-1 on NK-92 did not enhance cytotoxicity. Targeting the receptor on MM and LBCL did not also alter NK-92-mediated lysis. We come to the conclusion that LIR-1 alone does not directly influence NK-cell-mediated cytotoxicity against myeloma. To our knowledge, this work provides the first investigation of the inhibitory capability of LIR-1 in NK-92-mediated cytotoxicity against MM and the first functional evaluation of LIR-1 on MM and LBCL.
Authors:
Silke Heidenreich; Christine Zu Eulenburg; York Hildebrandt; Thomas Stübig; Heidi Sierich; Anita Badbaran; Thomas H Eiermann; Thomas M C Binder; Nicolaus Kröger
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Publication Detail:
Type:  Journal Article     Date:  2012-07-10
Journal Detail:
Title:  Clinical & developmental immunology     Volume:  2012     ISSN:  1740-2530     ISO Abbreviation:  Clin. Dev. Immunol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-30     Completed Date:  2013-01-31     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101183692     Medline TA:  Clin Dev Immunol     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  652130     Citation Subset:  IM    
Affiliation:
Clinic for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Blocking / immunology
Antibodies, Monoclonal / immunology
Antigens, CD / immunology*
COS Cells
Cell Line
Cercopithecus aethiops
Cytotoxicity, Immunologic*
Humans
Killer Cells, Natural / immunology*,  metabolism
Multiple Myeloma / immunology*
Receptors, Immunologic / antagonists & inhibitors*,  immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Blocking; 0/Antibodies, Monoclonal; 0/Antigens, CD; 0/LILRB1 protein, human; 0/Receptors, Immunologic
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