Document Detail


Impact of the menstrual cycle on circulating cell-free DNA.
MedLine Citation:
PMID:  20651374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: To determine serum levels of circulating cell-free DNA (cfDNA) throughout the stages of endometrial proliferation and apoptosis during the menstrual cycle. MATERIALS AND METHODS: cfDNA was measured in 176 blood samples from 17 healthy volunteers taken at three different time points (menstruation, follicular and secretory phase). Additionally, blood samples from 20 newly diagnosed breast cancer patients were analysed. Quantitative real-time PCR was performed in order to quantify cfDNA fragments of 106 bp. RESULTS: In healthy individuals, levels of cfDNA did not differ significantly during the menstrual cycle. In breast cancer patients, the median cfDNA level was significantly higher compared to healthy individuals, irrespective of the cycle phase (p<0.001). CONCLUSION: The female cycle does not influence cfDNA serum level measurements. Considering the diagnostic or prognostic value of cfDNA in cancer patients, different time points of blood sampling in premenopausal women seem to be negligible.
Authors:
Martin Pölcher; Jörg Ellinger; Stephanie Willems; Osman El-Maarri; Tobias Höller; Charlotte Amann; Matthias Wolfgarten; Christian Rudlowski; Walther Kuhn; Michael Braun
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-08-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2235-40     Citation Subset:  IM    
Affiliation:
Department of Gynecology and Obstetrics, Center for Integrated Oncology, Bonn University Medical Center, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany. martin.poelcher@ukb.uni-bonn.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers
Breast Neoplasms / blood*
DNA / blood*
Female
Humans
Menstrual Cycle*
Chemical
Reg. No./Substance:
0/Biological Markers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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