| Impact of High Fat and High Carbohydrate Diets on Liver Metabolism Studied in A Rat Model By A Systems Biology Approach. | |
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MedLine Citation:
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PMID: 22224854 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The aim of In the present study was to investigate the use of an integrated metabolomics and proteomics approach in the elucidation of diet-induced effects on hepatic metabolismthe impact of diets containing either a high level of fat or carbohydrate on the liver metabolome and the liver proteome was investigated in a rat model. Nuclear magnetic resonance (NMR)-based metabolomics of liver extracts revealed a pronounced effect of a high fat diet on the hepatic betaine content, while a carbohydrate-rich diet induced increases in hepatic glucose. In addition, the metabolomic investigations revealed that the high fat diet was associated with increased hepatic lipid levels, which was not evident upon the carbohydrate-rich diet. The proteomic investigations revealed strong high fat diet effects on the expression of 186numerous proteins in the liver including malate dehydrogenase. Comparison of malate dehydrogenase expression determined by proteomics and NMR metabolite profiles revealed correlations between malate dehydrogenase and lactate, glucose and glutamine/glutamate signals, thereby demonstrating a diet-induced regulation that was evident at both the proteomic and metabolomic level. |
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Authors:
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Hanne Christine Bertram; Lotte Bach Larsen; Xiaping Chen; Per Bendix Jeppesen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-19 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2012-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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