Document Detail

The Impact of Forced Joint Exercise on Lubricin Biosynthesis from Articular Cartilage Following ACL Transection and Intra-Articular Lubricin's Effect in Exercised Joints Following ACL Transection.
MedLine Citation:
PMID:  22579916     Owner:  NLM     Status:  Publisher    
OBJECTIVE: To evaluate the impact of forced joint exercise following acute knee injury on lubricin metabolism and its relationship to cartilage degeneration and to assess chondroprotection of a single-dose purified human lubricin injection in exercised injured joints. METHODS: Anterior cruciate ligament transection (ACLT) was performed in rats with six experimental groups; 3-week post-ACLT, 3-week post-ACLT + exercise, 5-week post-ACLT, 5-week post-ACLT + exercise, and 5-week post-ACLT + exercise treated with intra-articular PBS or lubricin. Joint exercise was achieved using a rotating cylinder at a speed of 6 rpm for 30 minutes daily, 5 days a week starting one week following surgery. Cartilage lubricin expression in injured joints was determined. Histological analyses included Safranin O/Fast Green, activated caspase-3, and lubricin mRNA in-situ hybridization. Assessment of cartilage damage was performed by OARSI modified Mankin scoring and urinary CTXII levels. RESULTS: At 3 weeks, lubricin expression in exercised ACLT joints was significantly (p<0.001) lower compared to ACLT joints. The OARSI scores were significantly (p<0.001) higher in the ACLT+exercise animals compared to ACLT animals at 5 weeks. Compared to 3-week ACLT, 3-week ACLT + exercise cartilage showed increased caspase-3 staining. Compared to ACLT+exercise and PBS-treated ACLT+exercise, lubricin intra-articular treatment resulted in a significant increase (p<0.001) in cartilage lubricin gene expression and a reduction (p<0.05) in uCTXII levels. CONCLUSION: Joint exercise resulted in decreased lubricin cartilage expression, increased cartilage degeneration and reduced superficial zone chondrocyte viability in the ACLT joint. Intra-articular lubricin administration ameliorated cartilage damage due to exercise and preserved superficial zone chondrocytes' viability.
K A Elsaid; L Zhang; K Waller; J Tofte; E Teeple; B C Fleming; G D Jay
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-8
Journal Detail:
Title:  Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society     Volume:  -     ISSN:  1522-9653     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9305697     Medline TA:  Osteoarthritis Cartilage     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, Boston, MA 02115.
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