Document Detail

Impact of carbon chain length on binding of perfluoroalkyl acids to bovine serum albumin determined by spectroscopic methods.
MedLine Citation:
PMID:  20397730     Owner:  NLM     Status:  MEDLINE    
Perfluoroalkyl acids (PFAAs), an emerging class of globally environmental contaminants, pose a great threat to humans with wide exposure from food and other potential sources. To evaluate the toxicity of PFAAs at the protein level, the effects of three PFAAs on bovine serum albumin (BSA) were characterized by fluorescence spectroscopy, synchronous fluorescence spectroscopy, and circular dichroism (CD). On the basis of the fluorescence spectra and CD data, we concluded that perfluoropentanoic acid (PFPA) had little effect on BSA. However, perfluorooctanoic acid (PFOA) and perfluorodecanoic acid (PFDA) exhibited remarkable fluorescence quenching, which was attributed to the formation of a moderately strong complex. The enthalpy change (DeltaH) and entropy change (DeltaS) indicated that van der Waals forces and hydrogen bonds were the dominant intermolecular forces in the binding of PFAAs to BSA. Furthermore, the BSA conformation was slightly altered in the presence of PFOA and PFDA, with a reduction of alpha helix. These results indicated that PFAAs indeed impact the conformation of BSA, and PFAAs with longer carbon chains were more toxic, especially at lower concentrations.
Pengfei Qin; Rutao Liu; Xingren Pan; Xiaoyan Fang; Yue Mou
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5561-7     Citation Subset:  IM    
School of Environmental Science and Engineering, Shandong University, Jinan, People's Republic of China.
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MeSH Terms
Acids / chemistry*
Carbon / chemistry*
Circular Dichroism
Fluorocarbons / chemistry*
Hydrogen Bonding
Serum Albumin, Bovine / chemistry*
Spectrometry, Fluorescence
Reg. No./Substance:
0/Acids; 0/Fluorocarbons; 0/Serum Albumin, Bovine; 7440-44-0/Carbon

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