Document Detail

Impact of Anesthetics On 3'-[18F]Fluoro-3'-Deoxythymidine ([18F]FLT) Uptake in Animal Models of Cancer and Inflammation.
MedLine Citation:
PMID:  23759369     Owner:  NLM     Status:  In-Data-Review    
AbstractThe aim of this study was to evaluate the impact of different anesthetics on 3'-[18F]fluoro-3'-deoxythymidine ([18F]FLT) uptake in carcinomas and arthritic ankles. To determine the amount of [18F]FLT uptake in subcutaneous CT26 colon carcinomas or arthritic ankles, spontaneously room air/medical air-breathing mice were anesthetized with isoflurane, a combination of medetomidine/midazolam, or ketamine/xylazine. Mice were kept conscious or anesthetized during [18F]FLT uptake before the 10-minute static positron emission tomographic (PET) investigations. [18F]FLT uptake in CT26 colon carcinomas and arthritic ankles was calculated by drawing regions of interest. We detected a significantly reduced (4.4 ± 0.9 %ID/cm3) [18F]FLT uptake in the carcinomas of ketamine/xylazine-anesthetized mice compared to the [18F]FLT-uptake in carcinomas of medetomidine/midazolam- (7.0 ± 1.5 %ID/cm3) or isoflurane-anesthetized mice (6.4 ± 1.5 %ID/cm3), whereas no significant differences were observed in arthritic ankles regardless of whether mice were anesthetized or conscious during tracer uptake. The time-activity curves of carcinomas and arthritic ankles yielded diverse [18F]FLT accumulation related to the used anesthetics. [18F]FLT uptake dynamics are different in arthritic ankles and carcinoma, and the magnitude and pharmacokinetics of [18F]FLT uptake are sensitive to anesthetics. Thus, for preclinical in vivo [18F]FLT PET studies in experimental tumor or inflammation models, we recommend the use of isoflurane anesthesia as it yields a stable tracer uptake and is easy to handle.
Kerstin Fuchs; Damaris Kukuk; Moritz Mahling; Leticia Quintanilla-Martinez; Gerald Reischl; Jörg Reutershan; Florian Lang; Martin Röcken; Bernd J Pichler; Manfred Kneilling
Related Documents :
8958309 - Differential histochemical peanut agglutinin stain in benign and malignant human prosta...
15877079 - Tumour-suppression activity of the proapoptotic regulator par4.
15586249 - Cytogenetic changes and loss of heterozygosity in atypical adenomatous hyperplasia, in ...
17332929 - Preferential association of prostate cancer cells expressing prostate specific membrane...
23492649 - Combining mtor inhibitors with chemotherapy and other targeted therapies in advanced br...
24367169 - Lapatinib: new opportunities for management of breast cancer.
10730569 - Digestive endoscopy: relevance of negative findings.
15287679 - Resistant starch and colorectal neoplasia.
17918159 - Naftopidil, a selective alpha-1 adrenoceptor antagonist, inhibits growth of human prost...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular imaging     Volume:  12     ISSN:  1536-0121     ISO Abbreviation:  Mol Imaging     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-06-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120118     Medline TA:  Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  277-87     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Earlier Diagnosis of Progressive Disease during Bevacizumab Treatment Using O-(2-18F-Fluorethyl)-L-t...
Next Document:  Improvement of in Vivo Quantification of [123I]-Iodobenzovesamicol in Single-Photon Emission Compute...