| Impact of 17beta-estradiol on cytokine-mediated apoptotic effects in primary hippocampal and neocortical cell cultures. | |
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MedLine Citation:
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PMID: 16949056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Estrogens are developmental regulators of mitochondrial apoptotic pathway in the central nervous system, but little is known about their involvement in cytokine-induced apoptosis. In the present study, we evaluated effects of 17beta-estradiol on pro-inflammatory cytokine- and staurosporine-mediated activation of caspase-3 and LDH-release in primary neuronal/glial cell cultures of mouse hippocampal and neocortical cells at different stages of their development in vitro. Enzyme activities were determined with colorimetric methods 6 h, 14 h, 24 h, and 48 h after exposure to the apoptotic agents. Biochemical data were supported at the cellular level by Hoechst 33342 and MAP-2 stainings, which were carried out 48 h after the treatment. Cytokines (co-treatment with Il-1beta and TNFalpha; 1 ng/ml) increased caspase-3 activity in the hippocampal and neocortical cells up to over 200% of control values, and these effects were mostly observed on 2 and 7 days in vitro (DIV). Moderate, but significant cytokine-mediated increase in LDH-release was demonstrated in both tissues, especially on 7 and 12 DIV. Estradiol (100 nM) inhibited the activation of caspase-3 at early stage of development (2 DIV) in the hippocampal, but not in the neocortical cultures. The cytokine-induced activation of caspase-3 and LDH-release was inhibited by estradiol in estrogen receptor-independent way. These data point to a possible role of estrogens as non-estrogen receptor-related inhibitors of cytokine-activated apoptotic pathway in the developing central nervous system. |
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Authors:
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Malgorzata Kajta; Andreas Trotter; Wladyslaw Lasoń; Cordian Beyer |
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Publication Detail:
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Type: Journal Article Date: 2006-09-01 |
Journal Detail:
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Title: Brain research Volume: 1116 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-09 Completed Date: 2006-12-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 64-74 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Cell Biology, University of Ulm, Albert-Einstein-Alle 11, 89061 Ulm, Germany. kajta@if-pan.krakow.pl |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Benzimidazoles Caspase 3 / metabolism Cells, Cultured Cytokines / pharmacology, physiology* Data Interpretation, Statistical Enzyme Inhibitors / pharmacology Estradiol / analogs & derivatives, pharmacology* Estrogen Antagonists / pharmacology Female Fluorescent Dyes Hippocampus / cytology, physiology* Interleukin-1beta / pharmacology L-Lactate Dehydrogenase / metabolism Mice Mice, Inbred BALB C Microtubule-Associated Proteins / drug effects, metabolism Neocortex / cytology, physiology* Neuroglia / drug effects, enzymology Neurons / drug effects, enzymology Pregnancy Staurosporine / pharmacology Tumor Necrosis Factor-alpha / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Benzimidazoles; 0/Cytokines; 0/Enzyme Inhibitors; 0/Estrogen Antagonists; 0/Fluorescent Dyes; 0/Interleukin-1beta; 0/Microtubule-Associated Proteins; 0/Tumor Necrosis Factor-alpha; 129453-61-8/fulvestrant; 23491-52-3/HOE 33342; 50-28-2/Estradiol; 62996-74-1/Staurosporine; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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