| Immunotoxicity of pyrethroid metabolites in an in vitro model. | |
| | |
MedLine Citation:
|
PMID: 20853454 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Risk assessment of man-made chemicals such as pesticides are mainly focused on parent compounds, and relatively little is known about their metabolites, especially with regard to target organ damages such as immunotoxicity. In the present study, the immunotoxicity of five synthetic pyrethroids (SPs) and three common metabolites was evaluated using an in vitro model by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cytoflow, and enzyme-linked immunosorbent assay (ELISA). Cell viability and apoptosis assays showed that both SPs and their metabolites possessed cytotoxicity to the monocytic cells. The aldehyde and acid derivatives were more effective than the other compounds at cytotoxicity, with inhibition of cell viability by 56.8 and 50.6% at 10⁻⁵ mol L⁻¹, and induction of 8.52 and 8.81% cell apoptosis, respectively. Exposure to SPs and their metabolites also led to changes in the secretion levels of tumor necrosis factor α (TNF α) and interleukins (ILs), and again the metabolites showed stronger effects than the parent compounds. The aldehyde derivative upregulated IL-12p70 level by 1.87-fold, and the alcohol and acid derivative increased the secretion of TNF α 5.88 and 7.96-fold, relative to the control group. In the in vitro model, the common metabolites of SPs clearly exerted greater immunotoxic effects to monocytes than the intact parent compounds. Results from the present study suggested the need for considering metabolites in achieving more comprehensive health risk assessment of man-made chemicals, including target organ toxicities such as immunotoxicity. |
| | |
Authors:
|
Ying Zhang; Meirong Zhao; Meiqing Jin; Chao Xu; Cui Wang; Weiping Liu |
Related Documents
:
|
15869864 - Expression of the nk-1 receptor on islet cells and invading immune cells in the non-obe... 16949684 - Signaling mechanisms of vasoactive intestinal peptide in inflammatory conditions. 7664654 - Vasoactive intestinal peptide-mediated suppression of apoptosis in the ovary: potential... 2812504 - Antibodies to calcitonin-gene related peptide reduce inflammation induced by topical mu... 9559974 - Heat shock proteins as potential targets in the therapy of inflammatory arthritis. 21562124 - Persistent activation of dendritic cells after resolution of allergic airway inflammati... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Environmental toxicology and chemistry / SETAC Volume: 29 ISSN: 1552-8618 ISO Abbreviation: Environ. Toxicol. Chem. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-10-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8308958 Medline TA: Environ Toxicol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 2505-10 Citation Subset: IM |
Copyright Information:
|
© 2010 SETAC. |
Affiliation:
|
MOE Key Lab of Environmental Remediation and Ecosystem Health, Zhejiang University, Hangzhou 310027, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Bioconcentration of perfluorinated compounds in blackrock fish, Sebastes schlegeli, at different sal...
Next Document: Linking toxicity in algal and bacterial assays with chemical analysis in passive samplers deployed i...