Document Detail


Immunosuppression by human seminal plasma--extracellular organelles (prostasomes) modulate activity of phagocytic cells.
MedLine Citation:
PMID:  1337434     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PROBLEM: Prostasomes are trilamellar to multilamellar vesicles produced by the acinar cells of the human prostate and are present in appreciable amounts in normal human semen. The aim of this work was to study the effect of prostasomes on human polymorphonuclear cell and monocyte function. METHODS: Functional activity of human neutrophils and monocytes was studied after incubation with prostasomes isolated from normal human seminal plasma. The following functional tests were employed: ability to ingest latex particles and opsonized bacteria (St. aureus) and ability to generate superoxide anion in response to PMA and FMLP. The latter was determined by measuring the superoxide-dismutase inhibitable reduction of ferricytochrome c to ferrocytochrome c at 550 nm. Expression of cell surface markers and interactions of prostasomes with cells were studied by cytofluorimetry. RESULTS: We show that prostasomes bind rapidly to the leukocyte cell membrane followed by internalization of adsorbed material. Interactions of prostasomes with neutrophils and monocytes inhibits their ability to phagocytose latex particles. The ability to ingest opsonized bacteria is, however, not impaired. Our results also show that incubation of leukocytes with prostasomes effectively inhibits superoxide anion generation in response to activation by PMA and FMLP. CONCLUSIONS: Prostasomes may play a complementary role to other immunosuppressive factors contained in the human semen. They may protect sperm cells from deleterious effects of phagocytosing cells, prolong their life, and consequently enhance the chance of conception. At the same time prostasomes may have a permissive effect on sexually transmitted diseases.
Authors:
G Skibinski; R W Kelly; D Harkiss; K James
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  28     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1993-03-17     Completed Date:  1993-03-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  97-103     Citation Subset:  IM    
Affiliation:
MRC Reproductive Biology Unit, University of Edinburgh, Scotland.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Membrane / immunology
Cells, Cultured
Dose-Response Relationship, Drug
Flow Cytometry
Humans
Immune Tolerance / physiology*
Latex / metabolism
Leukocytes / metabolism
Male
Monocytes / physiology
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Organelles / immunology*
Phagocytosis / physiology*
Prostate / cytology*
Receptors, Complement 3b / biosynthesis
Receptors, IgG / biosynthesis
Semen / cytology,  immunology*
Superoxides / metabolism
Tetradecanoylphorbol Acetate / pharmacology
Time Factors
Chemical
Reg. No./Substance:
0/Latex; 0/Receptors, Complement 3b; 0/Receptors, IgG; 11062-77-4/Superoxides; 16561-29-8/Tetradecanoylphorbol Acetate; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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