| Immunostimulation protects microglial cells from nitric oxide-mediated apoptosis. | |
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MedLine Citation:
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PMID:Â 9243619 Â Â Â Owner:Â NLM Â Â Â Status:Â MEDLINE Â Â Â |
Abstract/OtherAbstract:
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Cellular oxidative stress from excess free radicals can initiate apoptosis in some cell types. Thus, we hypothesize that cells expressing nitric oxide synthase (NOS) will also express anti-oxidative stress mechanisms, in order to prevent self-intoxication by the free radical, nitric oxide (NO). To test this hypothesis, we investigated the vulnerability of microglial cells (BV-2 line) to NO produced by the NO donor sodium nitroprusside. Damage to the cells was measured by an in situ detection method of DNA fragmentation, an indicator of apoptosis. Activation by lipopolysaccharide (LPS) dose-dependently protected BV-2 cells against NO toxicity (up to 67%) while unactivated BV-2 cells were vulnerable to NO. Our results indicate that activation of BV-2 cells induces protection mechanisms against NO toxicity. |
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Authors:
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K Sugaya; M Chouinard; M McKinney |
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Publication Detail:
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Type:Â Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Â Â Â |
Journal Detail:
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Title: Neuroreport    Volume: 8    ISSN: 0959-4965    ISO Abbreviation: Neuroreport    Publication Date: 1997 Jul |
Date Detail:
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Created Date:Â 1997-10-07 Â Â Â Completed Date:Â 1997-10-07 Â Â Â Revised Date:Â 2007-11-14 Â Â Â |
Medline Journal Info:
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Nlm Unique ID: 9100935    Medline TA: Neuroreport    Country: ENGLAND    |
Other Details:
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Languages: eng    Pagination: 2241-5    Citation Subset: IM    |
Affiliation:
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Department of Pharmacology, Mayo Clinic Jacksonville, FL 32224, USA. |
Export Citation:
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APA/MLA Format    Download EndNote    Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Cells, Cultured / drug effects Dose-Response Relationship, Drug Immunization* Lipopolysaccharides / pharmacology* Microglia / drug effects*, metabolism Nitric Oxide / toxicity* Nitrites / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AG09973/AG/NIA NIH HHS; AG12653/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/Nitrites; 10102-43-9/Nitric Oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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