Document Detail

Immunostaining of formalin-fixed, paraffin-embedded tissues for malignant lymphomas.
MedLine Citation:
PMID:  1304629     Owner:  NLM     Status:  MEDLINE    
With the advent of new monoclonal antibodies that are applicable to formalin-fixed, paraffin embedded sections, immunophenotyping is becoming increasingly important in the diagnosis and classification of lymphomas. However, multiple factors such as fixation, trypsinization and even type of antibodies used have certain effects on the final outcome of the staining procedure. In this paper we report our experience and the problems encountered in our laboratory when we first tried to establish a workable immunostaining protocol for formalin-fixed, paraffin embedded tissue sections using the immunoalkaline phosphatase technique.
Y C Lim; K S Phang; S K Cheong
Related Documents :
6178779 - Comparison of paired immunofluorescence and paired immunoenzyme staining methods based ...
1304629 - Immunostaining of formalin-fixed, paraffin-embedded tissues for malignant lymphomas.
8925599 - Accumulation of nuclear p53 protein and prognosis of astrocytomas in childhood and adol...
6349579 - Neurofilaments in adrenal and extra-adrenal pheochromocytoma. demonstration using immun...
17553519 - A novel automated method for enumeration of chlamydia trachomatis inclusion forming units.
8398549 - Notes on the application of microwaves for antigen retrieval in paraffin and plastic ti...
21062789 - Development of retinol-binding protein 4 immunocolloidal gold fast test strip using hig...
14598049 - Antibodies and myelination: facts and misacts.
2464579 - Immunological relationship among hydrogenases.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Malaysian journal of pathology     Volume:  14     ISSN:  0126-8635     ISO Abbreviation:  Malays J Pathol     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-07-08     Completed Date:  1993-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8101177     Medline TA:  Malays J Pathol     Country:  MALAYSIA    
Other Details:
Languages:  eng     Pagination:  85-9     Citation Subset:  IM    
Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Alkaline Phosphatase
Antibodies, Monoclonal
Lymphoma / pathology*
Paraffin Embedding*
Tissue Fixation / methods
Reg. No./Substance:
0/Antibodies, Monoclonal; 50-00-0/Formaldehyde; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  In situ hybridisation: principles and applications.
Next Document:  Alpha-1-antitrypsin deficiency in babies with prolonged jaundice.