Document Detail


Immunostaining of formalin-fixed, paraffin-embedded tissues for malignant lymphomas.
MedLine Citation:
PMID:  1304629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
With the advent of new monoclonal antibodies that are applicable to formalin-fixed, paraffin embedded sections, immunophenotyping is becoming increasingly important in the diagnosis and classification of lymphomas. However, multiple factors such as fixation, trypsinization and even type of antibodies used have certain effects on the final outcome of the staining procedure. In this paper we report our experience and the problems encountered in our laboratory when we first tried to establish a workable immunostaining protocol for formalin-fixed, paraffin embedded tissue sections using the immunoalkaline phosphatase technique.
Authors:
Y C Lim; K S Phang; S K Cheong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Malaysian journal of pathology     Volume:  14     ISSN:  0126-8635     ISO Abbreviation:  Malays J Pathol     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-07-08     Completed Date:  1993-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8101177     Medline TA:  Malays J Pathol     Country:  MALAYSIA    
Other Details:
Languages:  eng     Pagination:  85-9     Citation Subset:  IM    
Affiliation:
Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase
Antibodies, Monoclonal
Formaldehyde*
Humans
Immunohistochemistry
Lymphoma / pathology*
Paraffin Embedding*
Tissue Fixation / methods
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 50-00-0/Formaldehyde; EC 3.1.3.1/Alkaline Phosphatase

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