Document Detail

Immunosensor with a controlled orientation of antibodies by using NeutrAvidin-protein A complex at immunoaffinity layer.
MedLine Citation:
PMID:  16842878     Owner:  NLM     Status:  MEDLINE    
The orientation of antibody was controlled by using NeutrAvidin-protein A complex on the gold surface of SPR biosensor. The surface density of receptor antibody (anti-hIgG) was compared by treatment of receptor antibody to the layer of avidin, NeutrAvidin, protein A, NeutrAvidin-protein A complex and bare gold surface of SPR biosensor. The ligand antibody (hIgG) was injected to each IA layer and the binding ratio of ligand antibody per unit receptor was estimated as a parameter of orientation control. The NeutrAvidin-protein A complex on gold surface of SPR biosensor showed the highest surface density of receptor antibody as well as the binding ratio of ligand antibody per receptor antibody. The NeutrAvidin-protein A complex was also prepared on biotin-labelled SAM, and the binding ratio of ligand per receptor was found to be significantly improved in comparison to the IA layer prepared by chemical coupling of receptor antibody to the SAM layer. The NeutrAvidin-protein A complex which showed the highest efficiency for the binding of ligand antibodies, was applied for the detection of a cancer marker called CEA. By using NeutrAvidin-protein A complex and sandwich assay for signal amplification, sensitivity was improved to be 1.5-fold higher than bare gold surface and the detection of CEA with the detection limit of 30 ng/ml was achieved.
J W Chung; J M Park; R Bernhardt; J C Pyun
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-22
Journal Detail:
Title:  Journal of biotechnology     Volume:  126     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-18     Completed Date:  2006-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  325-33     Citation Subset:  IM    
Korea Institute for Science and Technology Europe (KIST Europe fGmbH), Stuhlsatzenhausweg 97, 66123 Saarbruecken, Germany.
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MeSH Terms
Antigen-Antibody Complex / analysis*,  immunology
Avidin / analysis*,  immunology*
Biosensing Techniques / instrumentation*,  methods
Coated Materials, Biocompatible / chemistry
Equipment Design
Equipment Failure Analysis
Immunoassay / instrumentation*,  methods
Staphylococcal Protein A / analysis*,  immunology*
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Coated Materials, Biocompatible; 0/Staphylococcal Protein A; 0/neutravidin; 1405-69-2/Avidin

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