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Immunosafety Evaluation of a Multilayer Flat-Plate Bioartificial Liver.
MedLine Citation:
PMID:  22008783     Owner:  NLM     Status:  Publisher    
INTRODUCTION:: To study and evaluate the immunosafety of our newly developed multilayer flat-plate bioartificial liver (BAL) in treatment of canines with acute liver failure. METHODS:: Fresh porcine hepatocytes and bone marrow mesenchymal stem cells were cocultured in new BAL. Ten canine models with acute liver failure were set up through d-galactosamine administration; 24 hours after administration, the beagles were randomly allocated to a 6-hour treatment with the BAL. The beagles were divided into 2 groups by treatment times. Group 1 beagles (n = 5) received a single BAL treatment. Group 2 beagles (n = 5) received 3 BAL treatments. The hemodynamic, hematologic response and humoral immune responses to BAL therapy were studied before and after treatments. RESULTS:: All beagles remained hemodynamically and hematologically stable during BAL treatments. The levels of IgG and IgM were similar before and after treatment after a single treatment. In addition, the level of CH50 in group 1 slightly decreased after the initiation of BAL treatment, and then the level recovered to baseline quickly after treatments. Time-course changes of the levels of antibodies and CH50 after 3 treatments in group 2 were similar to group 1. Only trace levels of IgG were detected in BAL medium after treatments. CONCLUSION:: The multilayer flat-plate BAL showed a great immunosafety in the treatment of canines with acute liver failure and exhibited a good prospect of its use in clinic.
Yue Zhang; Xiao-Lei Shi; Bing Han; Jin-Yang Gu; Xue-Hui Chu; Jiang-Qiang Xiao; Hao-Zhen Ren; Jiao-Jun Tan; Yi-Tao Ding
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-17
Journal Detail:
Title:  The American journal of the medical sciences     Volume:  -     ISSN:  1538-2990     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370506     Medline TA:  Am J Med Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
From the Department of Hepatobiliary Surgery (yz, x-ls, y-td), DrumTower Clinical Medical College of Nanjing Medical University; Department of Hepatobiliary Surgery (yz, x-ls, y-td, bh, j-yg, x-hc, j-qx, h-zr, j-jt), the Affiliated DrumTower Hospital of Nanjing University Medical School; and Jiangsu Province's Key Medical Center for Hepatobiliary Disease (yz, x-ls, y-td, bh, j-yg, x-hc, j-qx, h-zr, j-jt), Nanjing, China.
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