Document Detail


Immunoregulatory profile of monocytes from cutaneous leishmaniasis patients and association with lesion size.
MedLine Citation:
PMID:  23050581     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leishmaniasis is an important tropical disease composed of several clinical forms that adversely affect millions of people globally. Critical cells involved in the host-Leishmania interaction are monocytes and macrophages, which act to protect against infections due to their ability to both control intracellular infections and regulate the subsequent adaptive immune response. Both soluble factors and cell surface receptors are keys in directing the immune response following interaction with pathogens such as Leishmania. Toll-like receptors (TLRs) have an essential role in immune responses against infections, but little is known about their role in human infection with Leishmania braziliensis. In this work, we evaluated peripheral blood CD14+ monocytes for the expression of immunoregulatory cytokines, co-stimulatory molecules and TLR9 from cutaneous leishmaniasis patients infected with L. braziliensis and noninfected individuals. Our results showed that patients present decreased expression of co-stimulatory molecules such as CD80 and CD86 following culture with media alone or after stimulus with soluble Leishmania antigen. Interestingly, TLR9 expression was higher after culture with soluble Leishmania antigen (SLA), suggesting a role of this molecule in immunoregulation of active disease. Lastly, higher frequencies of TLR9+ monocytes were correlated with greater lesion size. These findings demonstrate a peripheral monocytes profile compatible with important immunoregulatory potential.
Authors:
É L M Vieira; T S L Keesen; P R Machado; L H Guimarães; E M Carvalho; W O Dutra; K J Gollob
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parasite immunology     Volume:  35     ISSN:  1365-3024     ISO Abbreviation:  Parasite Immunol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-10     Completed Date:  2013-06-13     Revised Date:  2014-11-06    
Medline Journal Info:
Nlm Unique ID:  7910948     Medline TA:  Parasite Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  65-72     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
Adaptive Immunity / immunology
Adolescent
Adult
Antigens, CD40 / blood
Antigens, CD80 / blood
Antigens, CD86 / blood
Antigens, Protozoan / immunology
Cytokines / blood
Flow Cytometry
Humans
Leishmania braziliensis / immunology*
Leishmaniasis, Cutaneous / blood,  immunology*,  parasitology,  pathology
Leukocytes, Mononuclear / immunology*,  pathology
Middle Aged
Monocytes / immunology*,  parasitology
Toll-Like Receptor 9 / blood,  immunology*
Young Adult
Grant Support
ID/Acronym/Agency:
AI066253/AI/NIAID NIH HHS; AI088650/AI/NIAID NIH HHS; R03 AI066253/AI/NIAID NIH HHS; U01 AI088650/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD40; 0/Antigens, CD80; 0/Antigens, CD86; 0/Antigens, Protozoan; 0/Cytokines; 0/TLR9 protein, human; 0/Toll-Like Receptor 9
Comments/Corrections

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