| Immunoreactive growth hormone-releasing factor in human stalk median eminence. | |
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MedLine Citation:
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PMID: 6427265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunoreactive growth hormone-releasing factor was detected in extracts of acetone preserved human stalk median eminence utilizing an antiserum against human GRF-(1-44)-NH2, isolated from a pancreatic tumor. The antibody is highly specific for the C-terminus of GRF-(1-44)-NH2, and has a cross-reactivity less than 0.02% with GRF-(1-40) free acid, a peptide isolated from a different pancreatic tumor. The mean concentration of IR-GRF in 5 pools of 10-15 stalk-median eminence fragments was 57.7 +/- 7.2 ng/mg protein (mean +/- SEM, range 42.5-75.8 ng/mg protein). Gel filtration chromatographic analysis showed that 97% of the IR-GRF coeluted with synthetic hpGRF-(1-44)-NH2 and eluate inhibition of binding in the radioimmunoassay was parallel to that of hpGRF-(1-44)-NH2. These data strongly suggest that human hypothalamic tissue contains a peptide that is similar to hpGRF-(1-44)-NH2. |
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Authors:
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H D Lin; J Bollinger; N Ling; S Reichlin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 58 ISSN: 0021-972X ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 1984 Jun |
Date Detail:
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Created Date: 1984-07-02 Completed Date: 1984-07-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1197-9 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gel Growth Hormone-Releasing Hormone / analysis* Humans Median Eminence / analysis* Radioimmunoassay |
| Chemical | |
Reg. No./Substance:
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9034-39-3/Growth Hormone-Releasing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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