Document Detail

Immunoradioautographic study of alpha-fetoprotein in hepatoma cells.
MedLine Citation:
PMID:  6158505     Owner:  NLM     Status:  MEDLINE    
Trypsin treatment, which is frequently used in the field of immunocytochemistry to faciliate conjugated antibody penetration through the cell membrane, was applied for the immunoradiographic demonstration of alpha-fetoprotein in ascites hepatoma cells using 125I-labeled anti rat alpha-fetoprotein antibody. No significant number of silver grains were detectable in the group without trypsin treatment, while numerous silver grains were recognizable in the ascites hepatoma cells after trypsin treatment. This indicates that trypsin treatment is effective for the immunoradiographic demonstration of alpha-fetoprotein and contributes to the quantitative immunoradioautographic investigation.
T Suganuma; M Koura; H Isaka; Y Tsukada; F Murata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histochemistry     Volume:  68     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1980  
Date Detail:
Created Date:  1980-12-18     Completed Date:  1980-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  129-32     Citation Subset:  IM    
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MeSH Terms
Ascites / immunology
Cell Line
Liver Neoplasms, Experimental / immunology
alpha-Fetoproteins / analysis*
Reg. No./Substance:

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