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Immunoproteomics and immunoinformatics analysis of Cryptococcus gattii: novel candidate antigens for diagnosis.
MedLine Citation:
PMID:  23534365     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Aim: To identify immunoreactive proteins of Cryptococcus gattii genotype VGII and their B-cell epitopes. Materials & methods: We combined 2D gel electrophoresis, immunoblotting and mass spectrometry to identify immunoreactive proteins from four strains of C. gattii genotype VGII (CG01, CG02, CG03 and R265). Next, we screened the identified proteins to map B-cell epitopes. Results: Sixty-eight immunoreactive proteins were identified. The strains and the number of proteins we found were: CG01 (12), CG02 (12), CG03 (18) and R265 (26). In addition, we mapped 374 peptides potentially targeted by B cells. Conclusion: Both immunoreactive proteins and B-cell epitopes of C. gattii genotype VGII that were potentially targeted by a host humoral response were identified. Considering the evolutionary relevance of the identified proteins, we may speculate that they could be used as the initial targets for recombinant protein and peptide synthesis aimed at the development of immunodiagnostic tools for cryptococcosis.
Authors:
Liline Maria Soares Martins; Hélida Monteiro de Andrade; Marilene Henning Vainstein; Bodo Wanke; Augusto Schrank; Claudia Bemfica Balaguez; Patrícia Ribeiro Dos Santos; Lucélia Santi; Simone da Fonseca Pires; Adalberto Socorro da Silva; José Adail Fonseca de Castro; Rafael Melo Santos de Serpa Brandão; Semiramis Jamil Hadad do Monte
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Future microbiology     Volume:  8     ISSN:  1746-0921     ISO Abbreviation:  Future Microbiol     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101278120     Medline TA:  Future Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  549-63     Citation Subset:  IM    
Affiliation:
Laboratório de Imunogenética e Biologia Molecular, Universidade Federal do Piauí, Campus Ministro Petrônio Portella Bloco SG-16, 64049-550, Teresina, Piauí, Brazil.
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