Document Detail


Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs.
MedLine Citation:
PMID:  21528460     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs. This strategy may also be used for immunopurification and directional sequencing of RNAs from other RNPs that contain small RNAs.
Authors:
Yohei Kirino; Anastassios Vourekas; Eugene Khandros; Zissimos Mourelatos
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  725     ISSN:  1940-6029     ISO Abbreviation:  -     Publication Date:  2011  
Date Detail:
Created Date:  2011-4-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  281-293     Citation Subset:  -    
Affiliation:
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