Document Detail

Immunophenotyping and extracellular matrix remodeling in pulmonary and extrapulmonary sarcoidosis.
MedLine Citation:
PMID:  22782602     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the significance of cellular immune markers, as well as that of collagen and elastic components of the extracellular matrix, within granulomatous structures in biopsies of patients with pulmonary or extrapulmonary sarcoidosis.
METHODS: We carried out qualitative and quantitative evaluations of inflammatory cells, collagen fibers, and elastic fibers in granulomatous structures in surgical biopsies of 40 patients with pulmonary and extrapulmonary sarcoidosis using histomorphometry, immunohistochemistry, picrosirius red staining, and Weigert's resorcin-fuchsin staining.
RESULTS: The extrapulmonary tissue biopsies presented significantly higher densities of lymphocytes, macrophages, and neutrophils than did the lung tissue biopsies. Pulmonary granulomas showed a significantly higher number of collagen fibers and a lower density of elastic fibers than did extrapulmonary granulomas. The amount of macrophages in the lung samples correlated with FVC (p < 0.05), whereas the amount of CD3+, CD4+, and CD8+ lymphocytes correlated with the FEV1/FVC ratio and VC. There were inverse correlations between TLC and the CD1a+ cell count (p < 0.05), as well as between DLCO and collagen/elastic fiber density (r = -0.90; p = 0.04).
CONCLUSIONS: Immunophenotyping and remodeling both showed differences between pulmonary and extrapulmonary sarcoidosis in terms of the characteristics of the biopsy samples. These differences correlated with the clinical and spirometric data obtained for the patients, suggesting that two different pathways are involved in the mechanism of antigen clearance, which was more effective in the lungs and lymph nodes.
Pedro Henrique Ramos Quintino da Silva; Edwin Roger Parra; William Sanches Zocolaro; Ivy Narde; Fabíola Rodrigues; Ronaldo Adib Kairalla; Carlos Roberto Ribeiro de Carvalho; Vera Luiza Capelozzi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia     Volume:  38     ISSN:  1806-3756     ISO Abbreviation:  J Bras Pneumol     Publication Date:    2012 May-Jun
Date Detail:
Created Date:  2012-07-11     Completed Date:  2012-11-20     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  101222274     Medline TA:  J Bras Pneumol     Country:  Brazil    
Other Details:
Languages:  eng; por     Pagination:  321-30     Citation Subset:  IM    
Universidade de São Paulo, São Paulo, SP, Brazil.
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MeSH Terms
Aged, 80 and over
Analysis of Variance
Collagen / immunology
Elastic Tissue / immunology,  pathology
Extracellular Matrix / immunology*,  pathology
Granuloma, Respiratory Tract / immunology,  pathology
Immunity, Cellular*
Immunophenotyping / methods*
Lung / immunology,  pathology
Lymph Nodes / immunology,  pathology
Lymphocytes / immunology
Macrophages, Alveolar / immunology
Middle Aged
Sarcoidosis / immunology*,  pathology
Sarcoidosis, Pulmonary / immunology,  pathology
Reg. No./Substance:

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