| Immunophenotypic stability of sezary cells by flow cytometry: usefulness of flow cytometry in assessing response to and guiding alemtuzumab therapy. | |
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MedLine Citation:
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PMID: 22338052 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Flow cytometry (FC) is frequently used to detect aberrant peripheral blood (PB) T cells ("Sézary cells") in patients with mycosis fungoides (MF) and Sézary syndrome (SS). However, immunophenotypic stability of MF/SS over time is not well characterized. We analyzed 141 PB samples from 9 cases (2 SS, 7 MF). At diagnosis, there were 3 to 5 immunophenotypic aberrancies per case (median, 4), including dim or absent CD2, CD3, CD4, CD5, CD7, or CD26 and bright CD45RO. Of 9 patients, 7 had a subsequent change in immunophenotype. All patients retained multiple aberrancies at follow-up (median, 3 per analysis; range, 2-6), of which 22.0% (81/369) were new. In 5 patients, a more than 99% decrease in absolute Sézary cell (ASC) counts by FC after alemtuzumab therapy or total skin electron beam radiation was associated with clinical improvement. We observed minor immunophenotypic changes over time in most patients with MF/SS; however, the Sézary clones maintain persistently aberrant immunophenotypes and seem amenable to follow-up with limited FC panels. ASC counts by FC correlated well with clinical response. |
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Authors:
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James Vaughan; Alexandra M Harrington; Parameswaran N Hari; Steven H Kroft; Horatiu Olteanu |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 137 ISSN: 1943-7722 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-02-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 403-11 Citation Subset: AIM; IM |
Affiliation:
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Dept of Pathology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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