Document Detail

Immunophenotypic analysis of cerebrospinal fluid cell populations with the Cell-Dyn Sapphire haematology analyser: method feasibility and preliminary observations.
MedLine Citation:
PMID:  19500178     Owner:  NLM     Status:  MEDLINE    
Cerebrospinal fluid (CSF) samples (n=50) from patients with neurological disease (bacterial infection, viral infection, neuroborreliosis and multiple sclerosis) were analysed to characterize cell populations by fluorescent immunocytometry with the CD-Sapphire haematology analyser. Reagent combinations applied to all CSF samples comprised CD3/CD19/HLA-DR and CD4/CD8, with some being further analysed using CD3/CD4, CD3/CD16 and CD3/CD25 protocols. Of the 50 samples, 11 were excluded because of high proportions of nonviable cells (n=2) or insufficient cell numbers (n=9). Apart from bacterial infection with granulocytosis, all diagnostic groups showed high proportions (51.4-77.0%) of CD3+ T cells. There was a modest association between T-cell and B-cell counts, but absolute B-cell numbers exceeded 5 cells/microl in only 7/39 cases (neuroborreliosis, n=6; bacterial meningitis, n=1). CD3/Ia antigen (activation) co-expression was low and only exceeded 5% in 7/39 samples with no diagnostic correlation. Primary CD4+ and CD8+ T-cell subsets showed similar quantitative trends and CD4/CD8 co-analysis revealed the presence in all diagnostic groups (neuroborreliosis and multiple sclerosis in particular) of a CD4+CD8int fraction that was predominantly CD3+ and CD16- and had a morphological profile consistent with small lymphoid cells. Supplementary CD-Sapphire cellular immunological analysis of most CSF samples is feasible using the procedure detailed in this communication.
P Adam; O Sobek; C S Scott; D Dolezil; J Kasik; L Hajdukova; D Adam
Related Documents :
7736738 - Alterations in immune function following head injury in children.
11454638 - Impaired th1 cytokine production in spondyloarthropathy is restored by anti-tnfalpha.
9008288 - Cd4+ t-cell lymphopenia in q fever endocarditis.
2572368 - Peripheral lymphocyte subsets and their responsiveness in human strongyloidiasis.
6973478 - Anti-thy-1-mediated t cell activation. role of soluble factors and expression of interl...
17898268 - Characterization of bone marrow derived cells in the substantia propria of the human co...
Publication Detail:
Type:  Journal Article     Date:  2009-06-01
Journal Detail:
Title:  International journal of laboratory hematology     Volume:  32     ISSN:  1751-553X     ISO Abbreviation:  Int J Lab Hematol     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-05-07     Completed Date:  2010-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101300213     Medline TA:  Int J Lab Hematol     Country:  England    
Other Details:
Languages:  eng     Pagination:  22-32     Citation Subset:  IM    
Institute of Cerebrospinal Fluid and Neuroimmunology, Central Military Hospital Complex, and Department of Neurology, Charles University in Prague, 3rd Medical School, Prague, Czech Republic.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cerebrospinal Fluid* / cytology,  immunology
Hematologic Tests / instrumentation,  methods
Immunophenotyping* / instrumentation,  methods
Nervous System Diseases / microbiology,  virology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Physical exercise is required for environmental enrichment to offset the quantitative effects of dar...
Next Document:  Quality of life for Korean patients with vitiligo: Skindex-29 and its correlation with clinical prof...