| Immunophenotypic analysis of anaplastic large cell lymphoma by flow cytometry. | |
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MedLine Citation:
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PMID: 12579990 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the antigen expression profiles of 19 anaplastic large cell lymphoma (ALCL) cases by multiparameter flow cytometry. The neoplastic cells expressed CD45, HLA-DR, and CD30 in all cases. At least 1 T cell-associated antigen was expressed in each case (CD2, 12/17 [71%]; CD4, 12/19 [63%]; CD3, 6/19 [32%]; CD7, 6/19 [32%]; CD5, 5/19 [26%]; CD8, 4/19 [21%]). CD25 was expressed in 14 (88%) of 16 cases. CD13 was expressed unexpectedly in 8 (47%) of 17 cases. One CD13+ ALCL also was positive for CD33 and 2 others for CD15, CD19, CD20, CD22, CD14, and CD36 were not expressed. Anaplastic lymphoma kinase protein was detected in about 33% (3/9) of ALCLs examined by flow cytometric immunophenotyping (FCI); expression was validated by immunohistochemical analysis. Of 19 ALCL cases, 12 were diagnosed solely based on FCI findings in conjunction with morphologic evaluation of body fluid (1 case), fine-needle aspirate (3 cases), or excisional biopsy specimen (8 cases). The diagnoses of the remaining 7 cases were suggested strongly by FCI and confirmed by immunohistochemical analysis. FCI is useful to aid in diagnosis of ALCL, particularly along with fine-needle aspiration evaluation. ALCL with aberrant expression of myeloid antigens should not be mistaken for extramedullary myeloid tumor. |
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Authors:
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Jonathan Juco; Jeannine T Holden; Karen P Mann; Lloyd G Kelley; Shiyong Li |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 119 ISSN: 0002-9173 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-12 Completed Date: 2003-02-26 Revised Date: 2011-06-08 |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 205-12 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Emory University Hospital, Room F143D, 1364 Clifton Rd NE, Atlanta, GA 30322, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Antigens, Neoplasm / immunology, metabolism Biopsy, Needle Child Child, Preschool Chromosome Banding Female Flow Cytometry* / methods Humans Immunohistochemistry Immunophenotyping* Lymph Nodes / immunology, metabolism, pathology Lymphoma, Large-Cell, Anaplastic / immunology*, metabolism, pathology* Male Middle Aged Protein-Tyrosine Kinases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/anaplastic lymphoma kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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