Document Detail


Immunophenotype of multinucleated and mononuclear cells in giant cell lesions of bone and soft tissue.
MedLine Citation:
PMID:  1597517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To compare the antigenic phenotype of giant cells in giant cell lesions of bone and soft tissue with that of osteoclasts and macrophage polykaryons. METHODS: Formalin fixed, paraffin wax embedded sections of 106 giant cell lesions, 19 granulomatous, and 14 osteoclast containing lesions were immuno-histochemically stained for leucocyte common antigen (LCA), CD68, and HLA-DR. RESULTS: Osteoclasts and giant cells of giant cell tumour of bone and giant cell reparative granuloma could be distinguished by their generalised absence of HLA-DR reaction from macrophage polykaryons and giant cells in other giant cell lesions of bone and soft tissue. Staining for LCA, CD68, and HLA-DR was useful in distinguishing reactive histiocytic giant cells and osteoclasts from tumour giant cells. CONCLUSIONS: A panel of macrophage associated antigens should be diagnostically useful in differentiating the histological nature of giant cells in various giant cell lesions of bone and soft tissue.
Authors:
I A Doussis; B Puddle; N A Athanasou
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  45     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-07-07     Completed Date:  1992-07-07     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  398-404     Citation Subset:  AIM; IM    
Affiliation:
Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, Oxford.
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MeSH Terms
Descriptor/Qualifier:
Bone Diseases / immunology,  pathology
Bone Neoplasms / immunology*,  pathology
Giant Cells / immunology*
HLA-DR Antigens / analysis
Humans
Immunoenzyme Techniques
Immunophenotyping
Macrophages / immunology*
Osteoclasts / immunology*
Soft Tissue Neoplasms / immunology*,  pathology
Chemical
Reg. No./Substance:
0/HLA-DR Antigens
Comments/Corrections
Comment In:
J Clin Pathol. 1993 Feb;46(2):190-1   [PMID:  8459047 ]

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