Document Detail


Immunopathological mechanisms of human T cell lymphotropic virus type 1 (HTLV-I) uveitis. Detection of HTLV-I-infected T cells in the eye and their constitutive cytokine production.
MedLine Citation:
PMID:  7860769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The immunopathology of human T cell lymphotropic virus type 1 (HTLV-I) uveitis was addressed by using T cell clones (TCC) established from the intraocular fluid of patients with HTLV-I uveitis. Proviral DNA of HTLV-I was identified in 55 out of 94 (59%) or 13 out of 36 (36%) TCC from the ocular fluid or the peripheral blood of these patients, respectively. Most of HTLV-I-infected TCC had a CD3+ CD4+ CD8- phenotype. HTLV-I infection on TCC was confirmed by analysis of the viral mRNA, nucleotide sequence, virus-associated proteins, and virus particles. HTLV-I-infected TCC, but not HTLV-I negative TCC, constitutively produced high amounts of IL-6 (1,336 +/- 1,050 pg/ml) and TNF-alpha (289 +/- 237 pg/ml) in the absence of any stimuli. HTLV-I-infected TCC from the ocular lesion also constitutively produced high amounts of IL-1 alpha (12,699 pg/ml), IL-2 (61 pg/ml), IL-3 (428 pg/ml), IL-8 (1,268 pg/ml), IL-10 (28 pg/ml), IFN-gamma (5,095 pg/ml), and GM-CSF (2,886 pg/ml). Hydrocortisone, a drug effective in vivo for the treatment of HTLV-I uveitis, severely depressed cytokine production in vitro in most cases. In summary, the results demonstrated direct evidence of HTLV-I infection of the eye and suggest that cytokines produced by HTLV-I-infected T cells are responsible for the intraocular inflammation in patients with HTLV-I uveitis.
Authors:
K Sagawa; M Mochizuki; K Masuoka; K Katagiri; T Katayama; T Maeda; A Tanimoto; S Sugita; T Watanabe; K Itoh
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  95     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-03-20     Completed Date:  1995-03-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  852-8     Citation Subset:  AIM; IM; X    
Affiliation:
Department of Immunology, Kurume University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aqueous Humor / immunology
Base Sequence
Behcet Syndrome / immunology,  pathology,  virology
Cytokines / biosynthesis*
DNA Primers
Female
Gene Expression / drug effects
Genes, env
Genes, pX
Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
HTLV-I Infections / immunology*,  pathology*,  virology
Human T-lymphotropic virus 1 / genetics,  isolation & purification*,  ultrastructure
Humans
Hydrocortisone / pharmacology
Interferon-gamma / biosynthesis
Interleukins / biosynthesis
Male
Microscopy, Electron
Middle Aged
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger / analysis
RNA, Viral / analysis
T-Lymphocytes / immunology*,  pathology
Uveitis / immunology*,  pathology*,  virology
Chemical
Reg. No./Substance:
0/Cytokines; 0/DNA Primers; 0/Interleukins; 0/RNA, Messenger; 0/RNA, Viral; 50-23-7/Hydrocortisone; 82115-62-6/Interferon-gamma; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor
Comments/Corrections

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