Document Detail

Immunopathogenesis of attenuated strain of chicken infectious anemia virus in one-day-old specific-pathogen-free chicks.
MedLine Citation:
PMID:  15726722     Owner:  NLM     Status:  MEDLINE    
In the present study, the immunopathogenicity of chicken anemia virus (CAV) vaccinal strain was studied in one-day-old specific-pathogen-free (SPF) chicks. Hematocrit values, histopathological changes in haemopioetic and lymphoid organs, ELISA for CAV antibodies and PCR for CAV genome were used as testing assays for the study. Vaccinated chicks showed signs of anemia, lower hematocrit values and histopathological lesions in liver in the form of hepatocytes swelling to Centro lobular necrosis and apoptosis. Histopathology change in spleen (depletion of lymphocytes and apoptosis) and thymus (depletion of thymocytes and apoptosis) together with variable degrees of seroconversion rate were observed along the 10 weeks of the experiment indicating 2 waves of immune response in vaccinated chicks compared to the control non-vaccinated group. Detection of CAV-DNA in the liver of vaccinated chicks indicated the presence of the virus, when the antibody levels were decreased in some chicks. There was a consistent correlation between the 4 parameters used. It is concluded that the attenuated CAV vaccine strain induces anemia and lesions in the lymphoid organs. The histopathology and PCR are useful tools for evaluation and quality assurance of CAV vaccines.
H A Hussein; M M Youssef; A Osman; E A El-Ebiary; M A Shalaby
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Egyptian journal of immunology / Egyptian Association of Immunologists     Volume:  10     ISSN:  1110-4902     ISO Abbreviation:  Egypt J Immunol     Publication Date:  2003  
Date Detail:
Created Date:  2005-02-21     Completed Date:  2005-03-18     Revised Date:  2008-02-11    
Medline Journal Info:
Nlm Unique ID:  9816016     Medline TA:  Egypt J Immunol     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  89-102     Citation Subset:  IM    
Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
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MeSH Terms
Anemia / etiology,  veterinary
Animals, Newborn
Antibodies, Viral / biosynthesis
Chicken anemia virus / genetics,  immunology*,  isolation & purification,  pathogenicity*
Circoviridae Infections / immunology,  prevention & control,  veterinary*
DNA, Viral / genetics,  isolation & purification
Liver / pathology,  virology
Poultry Diseases / immunology*,  prevention & control
Specific Pathogen-Free Organisms
Spleen / pathology
Thymus Gland / pathology
Vaccines, Attenuated / pharmacology,  toxicity
Viral Vaccines / pharmacology,  toxicity
Reg. No./Substance:
0/Antibodies, Viral; 0/DNA, Viral; 0/Vaccines, Attenuated; 0/Viral Vaccines

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