Document Detail


Immunonegative Staining: Epitope Localization on Macromolecules
MedLine Citation:
PMID:  8812676     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Relevant literature relating to immunonegative staining is reviewed and integrated with current research of the author and others. The immunonegative staining procedure has been utilized for the study of epitope localization on immune complexes formed from keyhole limpet hemocyanin type 2 (KLH2) di- and multidecamers, and the 20S and 26S proteasome from Xenopus laevis. The IgG linkage pattern of molecules in small immune complexes is considered to provide the most reliable indication of epitope location. For both KLH2 and the 20S proteasome, using domain-specific monoclonal antibodies and a 32-kDa (p32) subunit-specific polyclonal antibody, respectively, it is shown that epitopes (KLH2, subunit fg domain pair and the proteasome p32) are located on the ends of the two cylindrical molecules. Also, for KLH2 with a monoclonal antibody directed against subunit domain a, it is shown that this domain is located at the center of the KLH2 didecamer. Interpretation of the data for KLH2 indicates that the 10 subunits in each decamer are likely to be oriented in parallel. The epitopes of the 10 fg domain pairs are located on the ends, near the edge of the didecamer cylinder (i.e., at the "collar" region of the didecamer) and the epitope of the 10 a domains is located on the side wall of the didecamer cylinder, where the two decamers meet. In view of the varying shape of the Xenopus 20S proteasome immune complexes, it is tentatively proposed that there may be two p32 subunits located in each of the heptameric alpha rings rather than one, but the possibility of macromolecular heterogeneity with respect to p32 subunit composition cannot be discounted.
Authors:
Harris
Related Documents :
1281926 - Specific and sensitive igg4 immunodiagnosis of onchocerciasis with a recombinant 33 kd ...
3322616 - Immunoglobulin a1 and a2 subclass of salivary antibodies to candida albicans in patient...
6818676 - Triiodothyronine (t3) binding to immunoglobulin g subclasses in a slightly hypothyroid ...
2956496 - Demonstration of the existence of two distinct fc receptors for igg isotypes on guinea-...
7399566 - A rare blood group antigen, r1a (rosenlund).
20525276 - Use of monoclonal antibodies against hendra and nipah viruses in an antigen capture elisa.
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods (San Diego, Calif.)     Volume:  10     ISSN:  1095-9130     ISO Abbreviation:  Methods     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426302     Medline TA:  Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  234-46     Citation Subset:  -    
Affiliation:
Institute of Zoology, University of Mainz, Mainz, D-55099, Germany
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Bacterial Cell Wall Polymer-Induced Granulomatous Inflammation
Next Document:  Negative-Stain Immunoelectron-Microscopic Analysis of Small Macromolecules of Immunologic Significan...