Document Detail


Immunomodulatory effects of nisin in turbot (Scophthalmus maximus L.).
MedLine Citation:
PMID:  12526879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present work, the effect of nisin on the non-specific immune response of turbot (Scophthalmus maximus L.) leukocytes has been studied both in vitro and in vivo. The head kidney macrophage chemiluminescent (CL) response was significantly increased with intermediate doses of nisin (2.5 and 0.025 micro g ml(-1)) whilst the higher dose (25 micro g ml(-1)) significantly decreased the response after 24h incubation. When the incubation time was extended to 72 h, significant differences between doses were observed and the lower nisin concentration (0.025 micro g ml(-1)) appeared to be the optimum dose for increasing the CL response. The phagocytic activity of HK macrophages was also affected by in vitro nisin treatments. Nisin at 0.25 micro g ml(-1) and 0.025 micro g ml(-1) significantly stimulated the response after 24 and 72 h incubation respectively. Nitric oxide (NO) production by HK macrophages was not influenced by any nisin concentration employed for 24 or 72 h incubationsIn vivo, one week post injection, a slightly but non-significant stimulation of the CL response was observed with the lowest nisin concentration (0.0025 micro g fish(-1)). NO in serum and serum antibacterial index were not significantly affected by nisin treatments. On the other hand, lysozyme concentration in serum was significantly augmented with the lowest nisin dose (0.0025 micro g fish(-1)). The antibacterial effect of nisin against the fish pathogenic bacteria Carnobacterium piscicola (CECT 4020) was also demonstrated in vitro.
Authors:
L Villamil; A Figueras; B Novoa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  14     ISSN:  1050-4648     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-01-15     Completed Date:  2003-05-22     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  157-69     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones Marinas, CSIC, Eduardo Cabello 6, 36208, Vigo, Spain.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Anti-Bacterial Agents / immunology*,  pharmacology
Cells, Cultured
Chemiluminescent Measurements
Dose-Response Relationship, Immunologic
Flatfishes / immunology*
Kidney / cytology,  immunology
Leukocytes / drug effects,  immunology
Macrophages / drug effects*,  immunology
Nisin / immunology*,  pharmacology
Phagocytosis / drug effects
Time Factors
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 1414-45-5/Nisin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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