| Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica. | |
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MedLine Citation:
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PMID: 20645725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Morinda elliptica Ridley (Rubiaceae) has been used traditionally as a medicine to treat various diseases in Malaysia and southeast Asia. In the present study we investigated the immunomodulatory effects of damnacanthal isolated from the roots of Morinda elliptica. The immunomodulatory effect of this compound was evaluated by using the lymphocyte proliferation assay with mouse thymocytes and human peripheral blood mononuclear cells (PBMC). In addition, the effect of the compound on PBMC cell cycle progression was studied by using flow cytometry. The production of human interleukin-2 and human inteleukin-12 cytokines was also assessed using the enzyme linked immunosorbent assay (ELISA) technique. The lymphocyte proliferation assay showed that damnacanthal was able to activate mouse thymocytes and PBMC at a low concentration (0.468 microg/mL). Moreover, the production of human interleukin-2 and human interleukin-12 cytokines in the culture supernatant from damnacanthal activated lymphocytes was markedly up-regulated at 24 h and sustained until 72 h with a slight decrease with time. A positive correlation was found between the level of these two cytokines and the MTT-based proliferation assay. Based on the above results, damnacanthal can act as an immunomodulatory agent which may be very useful for maintaining a healthy immune system. |
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Authors:
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Noorjahan B Alitheen; A Abdul Manaf; S K Yeap; M Shuhaimi; L Nordin; A R Mashitoh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pharmaceutical biology Volume: 48 ISSN: 1744-5116 ISO Abbreviation: Pharm Biol Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-07-21 Completed Date: 2010-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9812552 Medline TA: Pharm Biol Country: England |
Other Details:
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Languages: eng Pagination: 446-52 Citation Subset: IM |
Affiliation:
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Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. noorjahan@biotech.upm.edu.my |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adjuvants, Immunologic
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isolation & purification,
pharmacology* Animals Anthraquinones / isolation & purification, pharmacology* Cell Proliferation / drug effects Culture Media Dose-Response Relationship, Drug Humans Interleukin-12 / biosynthesis, immunology Interleukin-2 / biosynthesis, immunology Leukocytes, Mononuclear / drug effects, immunology Lymphocyte Activation / drug effects, immunology Lymphocytes / drug effects, immunology Malaysia Male Mice Mice, Inbred ICR Morinda / chemistry* Plant Roots / chemistry Thymus Gland / cytology, drug effects, immunology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Adjuvants, Immunologic; 0/Anthraquinones; 0/Culture Media; 0/Interleukin-2; 187348-17-0/Interleukin-12; 477-84-9/damnacanthal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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