| Immunomodulation of monocyte-derived dendritic cells through ligation of tumor-produced mucins to Siglec-9. | |
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MedLine Citation:
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PMID: 20971061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dendritic cells (DCs) play an essential role in the induction and maintenance of an effective immune response and express multiple siglecs. In the present study, we investigated whether or not the ligation of tumor-produced mucins with Siglec-9 expressed on immature DCs is related to escape from immunosurveillance in the tumor-bearing state. Expression of Siglec-9 was up-regulated on the development of monocytes into immature DCs and was decreased in mature DCs. Binding of various mucins and artificial glycopolymers carrying poly (NeuAc α2,6 LacNAc) or poly (NeuAc α2,3 LacNAc) to Siglec-9 was demonstrated by means of a plate assay. These mucins also bound to the surface of immature DCs. When immature DCs were treated with LPS in the presence of these mucins or artificial glycopolymers, the production of IL-12 was significantly reduced, but that of IL-10 was not. Furthermore, IL-12 production was decreased to a similar level on treatment with anti-Siglec-9 mAb. Mucins prepared from serum of cancer patients actually could bind to Siglec-9. These results suggest that Siglec-9 expressed on DCs is involved in immunoregulation through ligation with mucins in an epithelial cancer patient. |
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Authors:
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Mariko Ohta; Akiko Ishida; Munetoyo Toda; Kaoru Akita; Mizue Inoue; Keishi Yamashita; Masashi Watanabe; Takeomi Murata; Taichi Usui; Hiroshi Nakada |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-29 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 402 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2011-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 663-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kita-ku, Kyoto, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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metabolism* Carcinoma / immunology* Cell Line, Tumor Dendritic Cells / immunology* Humans Immunomodulation Lectins / metabolism* Monocytes / immunology* Mucins / blood, metabolism* Neoplasms / immunology* Tumor Escape* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Lectins; 0/Mucins; 0/SIGLEC9 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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