| Immunomodulating activities of water-soluble exopolysaccharides obtained from submerged culture of Lentinus lepideus. | |
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MedLine Citation:
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PMID: 18756105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunomodulating activities of water-soluble exopolysaccharides (LL-EX) obtained from submerged mycelial culture of Lentinus lepideus were studied and their effectiveness was compared with lipopolysaccharide (LPS). The influence of the LL-EX on macrophage cellular lysosomal enzyme activity was to stimulate up to 267%, 392%, and 464% at the level of 10, 50, and 100 microg/ml, respectively. When the LL-EX was further fractionated into LL-Fr.I and Fr.II by Sepharose CL-6B gel chromatography, the cellular lysosomal enzyme activity of LL-Fr.II (2.1- fold) was higher than Fr.I (1.2-fold). Moreover, both LL-Fr.I and Fr.II stimulated the cytokines IL-1beta, TNF-alpha, and IL-6 in macrophages. In mixed lymphocyte reaction, LL-Fr.I and Fr.II enhanced the splenocyte proliferation up to 1.2-fold and 1.4-fold (50 microg/ml), respectively, stimulating only T lymphocytes. The fractions of LL-EX not show any direct toxicity against human gastric adenocarcinoma cell (AGS). The molecular masses of LL-Fr.I and Fr.II were estimated to be about 1,986 kDa and 21 kDa, respectively. The total sugar and protein contents of the two fractions were 84.97% and 69.88% and 15.03% and 30.12%, respectively. The sugar and amino acid compositions of the LL-Fr.I and Fr.II were also analyzed in detail. |
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Authors:
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Yu-Sun Jung; Byung-Keun Yang; Yong-Tae Jeong; Rezuanul Islam; Sang-Min Kim; Chi-Hyun Song |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of microbiology and biotechnology Volume: 18 ISSN: 1017-7825 ISO Abbreviation: J. Microbiol. Biotechnol. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-29 Completed Date: 2008-12-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9431852 Medline TA: J Microbiol Biotechnol Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 1431-8 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Daegu University, Gyeongsan 712-714, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / drug effects, immunology Cell Survival / drug effects, immunology Chromatography, Agarose Cytokines / biosynthesis Formazans / chemistry Humans Immunologic Factors / chemistry, isolation & purification*, pharmacology* Lentinula / chemistry* Lymphocyte Activation / drug effects Lymphocyte Culture Test, Mixed Macrophages / drug effects, enzymology Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Weight Polysaccharides / chemistry, isolation & purification*, pharmacology* Solubility Tetrazolium Salts / chemistry Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Formazans; 0/Immunologic Factors; 0/Polysaccharides; 0/Tetrazolium Salts; 23305-68-2/MTT formazan; 7732-18-5/Water |
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