Document Detail


Immunomagnetic cell enrichment detects more disseminated cancer cells than immunocytochemistry in vitro.
MedLine Citation:
PMID:  11025779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: We describe a method to improve tumor cell detection compared to currently available immunocytochemical methods by using immunomagnetic cell enrichment. MATERIALS AND METHODS: Two different methods of immunomagnetic cell enrichment using antibody coated magnetic beads were tested and compared with unenriched immunocytochemistry. One method was positive selection of epithelial cells from mononuclear cells with the antiepithelial antibody BER-EP4 and the other was depletion of mononuclear cells with the antileukocyte antibody CD45. Mononuclear cells were isolated from peripheral blood by density centrifugation and various numbers of tumor cells were added. The 5 different cell lines from urological malignancies used in the study were DU-145, RT-4, CAKI-2, KTCTL-2 and KTCTL-30. Following incubation of cell suspensions with the beads, cell separation was performed in a magnetic field. After centrifugation on glass slides immunocytochemical staining for cytokeratin was performed. A total of 112 experiments were completed and negative controls were obtained. RESULTS: The number of tumor cells detected by positive selection and depletion was significantly higher than by immunocytochemistry (p <0.001). The median enrichment factor and tumor cell recovery rate for positive selection and depletion were 15.3 and 61.2%, and 13.0 and 57.3%, respectively (not significant). With less than 1 tumor cell suspended in 106 mononuclear cells, the probability of tumor cell detection was 23% for immunocytochemistry alone and 93.3% for both enrichment methods (p <0.01). No false-positive results were observed. CONCLUSIONS: Compared to immunocytochemistry, immunomagnetic cell enrichment significantly improves the sensitivity of detection of epithelial cells added to mononuclear cells. Both methods of enrichment were equally effective and may be important for clinical practice in the future.
Authors:
R E Zigeuner; R Riesenberg; H Pohla; A Hofstetter; R Oberneder
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of urology     Volume:  164     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-11-21     Completed Date:  2000-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1834-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Urology, University of Graz, Graz, Austria.
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MeSH Terms
Descriptor/Qualifier:
Centrifugation, Density Gradient
Humans
Immunohistochemistry*
Immunomagnetic Separation / methods*
Leukocytes, Mononuclear
Sensitivity and Specificity
Tumor Cells, Cultured*
Urologic Neoplasms / pathology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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