Document Detail


Immunological studies on teleocalcin and salmon corpuscles of Stannius.
MedLine Citation:
PMID:  3129279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This report describes the characterization of an antiserum to salmon teleocalcin, a glycoprotein hormone that has recently been isolated from sockeye salmon, Oncorhynchus nerka, corpuscles of Stannius (CS). Immunodiffusion studies showed that teleocalcin was immunologically identical in four species of Pacific salmon. Histological staining of adjacent sections of sockeye salmon CS by periodic acid-Schiff reagent and immunocytochemistry revealed that the teleocalcin-immunoreactive cells were also periodic acid-Schiff positive. In bioassays using rainbow trout (Salmo gairdneri), the biological effects of teleocalcin were completely abolished by pretreatment with the antiserum before injection. The antiserum was ineffective by itself, however, in neutralizing endogenous teleocalcin. Immunoprecipitation studies of in vitro translation products from salmon CS poly(A)+ RNA identified a 26K protein, which corresponds to the size of the teleocalcin monomer as predicted by cDNA sequencing studies. Similar banding patterns were obtained with purified teleocalcin and crude CS extracts by Western blot analysis. However, there was one extra band (32K) in the CS extracts that was not present in the purified hormone preparations. This may represent the pro-form of the hormone in salmon.
Authors:
G F Wagner; D H Copp; H G Friesen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  122     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-06-01     Completed Date:  1988-06-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2064-70     Citation Subset:  AIM; IM    
Affiliation:
Department of Physiology, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Glycoproteins / analysis*,  genetics,  immunology
Hormones*
Immunodiffusion
Immunohistochemistry
Male
Molecular Weight
Protein Biosynthesis
Salmon / metabolism*
Species Specificity
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Hormones; 76687-96-2/teleocalcin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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