Document Detail


Immunological properties of rat phosphoglycerate mutase isozymes.
MedLine Citation:
PMID:  2844269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In mammalian tissues three phosphoglycerate mutase (D-phosphoglycerate 2,3-phosphomutase, EC 5.4.2.1) isozymes result from the homo-dimeric and hetero-dimeric combinations of two subunits (types M and B). Whereas rabbit antisera against type M subunit (purified from rat muscle) and against type BB isozyme (purified from rat brain) possessed a high degree of specificity, both antisera reacted with type BB and MM isozymes, as demonstrated by immunoneutralization and ELISA. Both the M subunit and B subunit were more immunoreactive than their respective dimeric isozymes. Subunits type M and B may possess common antigenic determinants, and some of these determinants may be sterically hindered in their dimeric structures.
Authors:
J Castellá; J Ureña; D Ludevid; J Carreras; F Climent
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  956     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-11-23     Completed Date:  1988-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  97-102     Citation Subset:  IM    
Affiliation:
Unitat de Bioquímica, Facultat de Medicina, Universitat de Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bisphosphoglycerate Mutase / immunology*
Enzyme-Linked Immunosorbent Assay
Isoenzymes / immunology*
Molecular Weight
Phosphotransferases / immunology*
Rats
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 2.7.-/Phosphotransferases; EC 5.4.2.4/Bisphosphoglycerate Mutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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