Document Detail

Immunological investigation of the hepatic tissue from infants with biliary atresia.
MedLine Citation:
PMID:  19089432     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Matrix metalloproteinases (MMPs) and their endogenous tissue inhibitors [tissue inhibitors of metalloproteinases (TIMPs)] have been implicated in tissue injury and remodeling in many organs. The objective of this study was to evaluate the expression of MMP-3 and -9, and TIMP-1, -2, and -3 and their relationship to liver fibrosis in infants with biliary atresia. METHODS: The expression of MMP-3 and-9 and TIMP-1, -2 and -3 was investigated in liver tissue samples of nine patients with biliary atresia. In addition, the expression of CCR-4 and CCR-5 was analyzed to investigate the activation of Th1 and Th2 cells. The mRNA levels were measured by semiquantitative reverse transcriptase polymerase chain reaction. RESULTS: The expression of MMP-3 was higher than that of MMP-9 in all samples (P < 0.01). The expression of TIMP-1 was higher than that of TIMP-2 and -3 in all samples (P < 0.01). The expression of CCR-5 was higher than that of CCR-4 (P < 0.05), which implied higher activation of Th1 cells relative to Th2 cells. CONCLUSION: Our findings suggest that MMP-3, possibly induced by Th1 cytokines, and its balance with TIMP-1, may be one of the factors involved in the pathogenesis of biliary atresia.
Haruna Baba; Yoshikazu Ohtsuka; Tohru Fujii; Hidenori Haruna; Satoru Nagata; Hiroyuki Kobayashi; Atsuyuki Yamataka; Toshiaki Shimizu; Takeshi Miyano; Yuichiro Yamashiro
Publication Detail:
Type:  Journal Article     Date:  2008-12-17
Journal Detail:
Title:  Pediatric surgery international     Volume:  25     ISSN:  1437-9813     ISO Abbreviation:  Pediatr. Surg. Int.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-23     Completed Date:  2009-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609169     Medline TA:  Pediatr Surg Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  157-62     Citation Subset:  IM    
Department of Pediatrics and Adolescence Medicine, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
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MeSH Terms
Biliary Atresia / complications,  immunology*
Infant, Newborn
Liver / immunology*
Liver Cirrhosis / immunology*,  metabolism
Matrix Metalloproteinase 3 / biosynthesis
Matrix Metalloproteinase 9 / biosynthesis
Receptors, CCR4 / biosynthesis
Receptors, CCR5 / biosynthesis
Tissue Inhibitor of Metalloproteinases / biosynthesis
Reg. No./Substance:
0/Receptors, CCR4; 0/Receptors, CCR5; 0/Tissue Inhibitor of Metalloproteinases; EC Metalloproteinase 3; EC Metalloproteinase 9

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