| Immunological identity of a 60 kd oncofetal protein induced in rats by chemical carcinogens and released by transformed cells. | |
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MedLine Citation:
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PMID: 2580518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A 60,000 dalton (60 kd) oncofetal protein was previously shown to be produced by tumors in tumor-bearing rats and by target tissues within 3 weeks of carcinogen treatment. The factor is released to and accumulates in the blood in vivo and in the conditioned medium of cultured transformed cells in vitro. A polyclonal antibody produced against the 60 kd factor purified from the plasma of a rat carrying the N-2-fluorenylphthalamic acid-induced transplantable Hepatoma 7777, was tested against the 60 kd factor from various sources. Based on the results of immunoprecipitation of biochemical activity associated with the 60 kd factor, it was determined that these anti-60 kd antibodies cross-reacted with the factor released by a dimethylbenzanthracene-induced rat mammary carcinoma, with the factor in rat tumor cytosol and with rat spontaneous lymphoma cells, but not with a 60 kd factor isolated from pooled cancer patient plasma. Furthermore, these antibodies cross-reacted with the 60 kd factor induced within 21 days of treatment of the rats with a range of carcinogens from 8 chemical structural groups. The anti-60 kd factor antibodies did not cross-react with a 35 kd factor having similar biochemical activity found in normal adult cells. |
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Authors:
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M Hanausek-Walaszek; R W Lang; Z Walaszek; T E Webb |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 127 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1985 Mar |
Date Detail:
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Created Date: 1985-05-01 Completed Date: 1985-05-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 779-85 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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9,10-Dimethyl-1,2-benzanthracene Animals Antibodies / immunology Antigens, Neoplasm / immunology Carcinogens / pharmacology* Cell Transformation, Neoplastic / metabolism* Epitopes / immunology Female Immunosorbent Techniques Liver Neoplasms, Experimental / chemically induced, metabolism Mammary Neoplasms, Experimental / chemically induced, metabolism Neoplasm Transplantation Rats Rats, Inbred BUF Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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CA 30627/CA/NCI NIH HHS; P-30-CA-16058-09/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Antigens, Neoplasm; 0/Carcinogens; 0/Epitopes; 0/oncofetal antigens; 57-97-6/9,10-Dimethyl-1,2-benzanthracene |
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