Document Detail

Immunological development of preterm infants in early infancy.
MedLine Citation:
PMID:  15762879     Owner:  NLM     Status:  MEDLINE    
To evaluate the immunological development of preterm infants, especially in early infancy, we examined the serum cytokine levels and the expression of Th2 and Th1 chemokine receptors, CCR4 and CCR5, on days 0, 14 and 28 in 16 low birth weight infants (1720.38 +/- 502.80 g) born at less than 37 (33.63 +/- 3.29) weeks of gestation. Using an enzyme-linked immunosorbent assay (ELISA), serum interleukin (IL)-4 levels exhibited an increase on day 14, but decreased to the initial level on day 28 (P < 0.05). The significant elevation of serum transforming growth factor (TGF)-beta levels was confirmed on day 14 (P < 0.05) but decreased to the initial level on day 28 (P < 0.05). The expression of CCR4 and CCR5 were examined using reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometric analysis. The RT-PCR confirmed the expression of CCR5-mRNA soon after birth, while there was no expression of CCR4-mRNA. Thereafter, the expression of CCR4-mRNA increased significantly and reached the level of CCR5-mRNA expression on day 28 (P < 0.05). Flow cytometric analysis, however, revealed that the expression levels of both CCR4 and CCR5 were low at birth. Thus, CCR4(+) CD4(+) cells were significantly increased from days 0-28 (P < 0.05), while CCR5(+) CD4(+) cells were not. Increased IL-4 and TGF-beta synthesis as well as increased CCR4(+) CD4(+) cells suggest that, under extra-maternal circumstances, there is a shift in bias toward Th2 responses even in preterm infants soon after delivery, while they may be capable of developing Th1 mediated responses soon after birth.
B Zhang; Y Ohtsuka; T Fujii; H Baba; K Okada; H Shoji; S Nagata; T Shimizu; Y Yamashiro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  140     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-14     Completed Date:  2005-05-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  92-6     Citation Subset:  IM    
Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
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MeSH Terms
Actins / analysis
Biological Markers / analysis
Chemokines, CC / immunology
Cytokines / blood*
Infant, Low Birth Weight / immunology*
Infant, Newborn
Infant, Premature / immunology*
RNA, Messenger / analysis
Receptors, CCR4
Receptors, CCR5 / analysis*
Receptors, CCR8
Receptors, CXCR3
Receptors, Chemokine / analysis*
Reverse Transcriptase Polymerase Chain Reaction / methods
Th1 Cells / immunology
Th2 Cells / immunology
Reg. No./Substance:
0/Actins; 0/Biological Markers; 0/CCR4 protein, human; 0/CCR8 protein, human; 0/CXCR3 protein, human; 0/Chemokines, CC; 0/Cytokines; 0/RNA, Messenger; 0/Receptors, CCR4; 0/Receptors, CCR5; 0/Receptors, CCR8; 0/Receptors, CXCR3; 0/Receptors, Chemokine

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