| Immunological comparison of constitutive beta-lactamases of gram-negative bacteria by neutralization in zymogram gels: properties of anti-TEM-1 and anti-TEM-2 sera. | |
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MedLine Citation:
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PMID: 2460115 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The zymogram technique was applied to a beta-lactamase neutralization assay with anti-TEM-1 and anti-TEM-2 sera. Both were shown to contain neutralizing antibodies directed towards various beta-lactamases of Gram-negative bacteria. The quantitative neutralization allowed classification into five groups of the 28 beta-lactamases used as standards and 61 from clinical isolates. In the first were enzymes such as TEM-1 and TEM-2 including TLE-1, SHV-1, SHV-2, penicillinases of Klebsiella pneumoniae and CTX-1. Partial neutralization distinguished two groups containing the CARB group of enzymes, which are different from PSE-2 and PSE-3, and the MAL penicillinases of Levinea malonatica, which are different from L. amalonatica enzymes. Broad spectrum beta-lactamases of K. oxytoca constituted a unique group of partially neutralized enzymes. Among the beta-lactamases not neutralized by either serum were the plasmid-mediated OXA-enzymes, various species-specific beta-lactamases and cephalosporinases. The antigenic similarities of the enzymes appeared to correlate with the extent of similarities of their catalytic properties, namely those of penicillinases. Such comparisons between the beta-lactamase groups provide an indirect approach to the physiological and structural analysis of established and recently evolved beta-lactamases. |
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Authors:
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G Paul; M Barthélémy; A Philippon; J Peduzzi; L Gilly; R Labia; P Névot |
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Publication Detail:
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Type: Comparative Study; Journal Article; Review |
Journal Detail:
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Title: Annales de l'Institut Pasteur. Microbiology Volume: 139 ISSN: 0769-2609 ISO Abbreviation: Ann. Inst. Pasteur Microbiol. Publication Date: 1988 Jul-Aug |
Date Detail:
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Created Date: 1988-12-20 Completed Date: 1988-12-20 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 8701984 Medline TA: Ann Inst Pasteur Microbiol Country: FRANCE |
Other Details:
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Languages: eng Pagination: 435-51 Citation Subset: IM |
Affiliation:
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Service de Bactériologie, UER Cochin-Port-Royal, Paris. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Epitopes
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immunology* Gram-Negative Bacteria / enzymology* Immune Sera / immunology* Immunoenzyme Techniques beta-Lactamases / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Epitopes; 0/Immune Sera; EC 3.5.2.6/beta-Lactamases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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