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Immunological characterization of a major transformation-sensitive fibroblast cell surface glycoprotein. Localization, redistribution, and role in cell shape.
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MedLine Citation:
PMID:  357438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The major cell surface glycoprotein of chick embryo fibroblasts, cellular fibronectin (formerly known as CSP or LETS protein), was purified and used to produce monospecific antisera. After affinity purification, the anti-fibronectin was used to investigate fibronectin's localization, its transfer from intracellular to extracellular pools, its antibody-induced redistribution on the cell surface, and its role in cell shape. Anti-fibronectin localizes to extracellular fibrils located under and between sparse cells, and to a dense matrix that surrounds confluent cells. Cellular fibronectin is also present in granular intracytoplasmic structures containing newly synthesized fibronectin before secretion. This intracellular staining disappears 2 h after treatment with cycloheximide or puromycin, and returns after removal of these protein synthesis inhibitors. In pulse-chase experiments using cycloheximide, fibronectin was sequentially transferred from the intracellular to the fibrillar extracellular forms. Transformation of chick fibroblasts results in decreases in both extracellular and intracellular fibronectin, and in altered cell shape. Treatment of untransformed chick fibroblasts with anti-fibronectin results in rapid (30 min) alteration to a rounder cell shape resembling that of many transformed cells. These rapid shape changes are followed by a slow, antibody-induced redistribution of fibronectin to supranuclear caplike structures. This "capping" is inhibited by metabolic inhibitors. Reconstitution of cell surface fibronectin onto transformed cells restores a more normal fibroblastic phenotype. The reconstituted fibronectin on these cells organizes into fibrillar patterns similar to those of untransformed cells. As with untransformed cells, treatment of these reconstituted cells with anti-fibronectin also results in cell rounding and "capping" of fibronectin.
Authors:
K M Yamada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  78     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1978 Aug 
Date Detail:
Created Date:  1978-12-02     Completed Date:  1978-12-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  520-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Membrane / ultrastructure
Cell Transformation, Neoplastic*
Chick Embryo
Cycloheximide / pharmacology
Fibroblasts / cytology,  immunology,  ultrastructure*
Fluorescent Antibody Technique
Glycoproteins / immunology*,  isolation & purification
Immune Sera
Immunologic Capping
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Immune Sera; 66-81-9/Cycloheximide
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 8 Year: 1978
Volume: 78 Issue: 2
First Page: 520 Last Page: 541
ID: 2110125
Publisher Id: 79005759
PubMed Id: 357438

Immunological characterization of a major transformation-sensitive fibroblast cell surface glycoprotein. Localization, redistribution, and role in cell shape


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