Document Detail

Immunological analysis of phloem sap of Bacillus thuringiensis corn and of the nontarget herbivore Rhopalosiphum padi (Homoptera: Aphididae) for the presence of Cry1Ab.
MedLine Citation:
PMID:  11298965     Owner:  NLM     Status:  MEDLINE    
Phloem sap of transgenic Bacillus thuringiensis (Bt) corn expressing a truncated form of the B. thuringiensis delta-endotoxin Cry1Ab, sap sucking aphids feeding on Bt corn and their honeydew were analysed for presence of Cry1Ab using ELISA. Phloem sap of Bt and non-Bt corn was collected using a newly developed technique with a microcapillary being directly inserted into the phloem tubes. Using this technique, no Cry1Ab was detected in the phloem sap. In contrast, measurable concentrations of Cry1Ab in the range of 1 ppb were detected when phloem sap of pooled leaf samples was extracted using EDTA buffer. This was probably because of Cry1Ab toxin released from damaged cells. When analysing apterous adults of Rhopalosiphum padi L. and their honeydew, no Cry1Ab could be detected. In contrast, Cry1Ab was clearly detected in both larvae of the leaf chewing herbivore Spodoptera littoralis (Boisduval) and their faeces, showing that Cry1Ab is detectable after ingestion and excretion by herbivores. These results suggest that R. padi ingests or contains no or only very low concentrations of Cry1Ab in the range of the detection limit. In consequence it is hypothesized that R. padi as an important prey for beneficial insects in corn is unlikely to cause any harm to its antagonists due to mediating Bt toxin.
A Raps; J Kehr; P Gugerli; W J Moar; F Bigler; A Hilbeck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular ecology     Volume:  10     ISSN:  0962-1083     ISO Abbreviation:  Mol. Ecol.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-04-12     Completed Date:  2001-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9214478     Medline TA:  Mol Ecol     Country:  England    
Other Details:
Languages:  eng     Pagination:  525-33     Citation Subset:  IM    
Swiss Federal Research Station of Agroecology and Agriculture, CH-8046 Zürich, Switzerland.
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MeSH Terms
Aphids / chemistry*,  physiology
Bacillus thuringiensis / enzymology,  genetics*,  immunology
Bacterial Proteins / analysis*,  genetics,  immunology
Bacterial Toxins / analysis*,  genetics,  immunology
Endotoxins / analysis*,  genetics,  immunology
Enzyme-Linked Immunosorbent Assay
Hemolysin Proteins
Larva / chemistry,  physiology
Moths / physiology
Plant Leaves / chemistry,  immunology,  parasitology
Plants, Genetically Modified / chemistry,  genetics*,  immunology
Zea mays / chemistry*,  parasitology
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacterial Toxins; 0/Endotoxins; 0/Hemolysin Proteins; 0/insecticidal crystal protein, Bacillus Thuringiensis

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