Document Detail

Immunologic response to protein immobilized on the surface of liposomes via covalent azo-bonding.
MedLine Citation:
PMID:  6547057     Owner:  NLM     Status:  MEDLINE    
A new method for immobilizing protein on the surface of liposomes is described. Inclusion of N-(p-aminophenyl) stearylamide in the lipid composition of vesicles resulted in liposomes that could be 'activated' by diazotization with NaNO2/HCl, and subsequently coupled with protein. Using this method 39.7 +/- 7.5 micrograms egg albumin/mumol phospholipid has been coupled to multilamellar vesicles composed of phosphatidylcholine, cholesterol, and N-(p-aminophenyl) stearylamide in a molar ratio of 15:75:1.1. Furthermore, when the immunologic response of mice to egg albumin that was encapsulated in, nonspecifically absorbed, or covalently linked to liposomes was investigated, only the covalent protein-liposome conjugates elicited pronounced and sustained elevations in antibody titers. These results suggest that the immunoadjuvant effects of liposomes can be maximized by covalently linking protein antigens to their surface.
S L Snyder; W E Vannier
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  772     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-07-16     Completed Date:  1984-07-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  288-94     Citation Subset:  IM    
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MeSH Terms
Albumins / immunology
Antibody Formation
Antigens / immunology*
Azo Compounds*
Chemical Phenomena
Membrane Lipids
Proteins / immunology*
Stearic Acids / diagnostic use
Reg. No./Substance:
0/Albumins; 0/Antigens; 0/Azo Compounds; 0/Liposomes; 0/Membrane Lipids; 0/Proteins; 0/Stearic Acids; 91608-14-9/N-(4-aminophenyl)stearylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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