Document Detail


Immunologic response to protein immobilized on the surface of liposomes via covalent azo-bonding.
MedLine Citation:
PMID:  6547057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new method for immobilizing protein on the surface of liposomes is described. Inclusion of N-(p-aminophenyl) stearylamide in the lipid composition of vesicles resulted in liposomes that could be 'activated' by diazotization with NaNO2/HCl, and subsequently coupled with protein. Using this method 39.7 +/- 7.5 micrograms egg albumin/mumol phospholipid has been coupled to multilamellar vesicles composed of phosphatidylcholine, cholesterol, and N-(p-aminophenyl) stearylamide in a molar ratio of 15:75:1.1. Furthermore, when the immunologic response of mice to egg albumin that was encapsulated in, nonspecifically absorbed, or covalently linked to liposomes was investigated, only the covalent protein-liposome conjugates elicited pronounced and sustained elevations in antibody titers. These results suggest that the immunoadjuvant effects of liposomes can be maximized by covalently linking protein antigens to their surface.
Authors:
S L Snyder; W E Vannier
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  772     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-07-16     Completed Date:  1984-07-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  288-94     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Albumins / immunology
Animals
Antibody Formation
Antigens / immunology*
Azo Compounds*
Chemical Phenomena
Chemistry
Female
Liposomes*
Membrane Lipids
Mice
Proteins / immunology*
Stearic Acids / diagnostic use
Chemical
Reg. No./Substance:
0/Albumins; 0/Antigens; 0/Azo Compounds; 0/Liposomes; 0/Membrane Lipids; 0/Proteins; 0/Stearic Acids; 91608-14-9/N-(4-aminophenyl)stearylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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