Document Detail


Immunologic characterization and specificity of three monoclonal antibodies against the 58-kilodalton protein of Legionella pneumophila.
MedLine Citation:
PMID:  1890189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three monoclonal antibodies against the Legionella pneumophila 58-kDa protein were produced. By using immunoblot analysis, the percentages of reactivity against 47 serogroups of Legionella representing 29 species were determined to be 80.9, 87.2, and 95.6 for monoclonal antibodies GB5BE8, GB5AF6, and CA4AF5, respectively. Specificities obtained from testing 63 heterologous organisms representing 22 genera and 46 species were 90.7, 92.2, and 95.3% for monoclonal antibodies GB5BE8, GB5AF6, and CA4AF5, respectively. No single heterologous strain was reactive with all three monoclonal antibodies. These monoclonal antibodies successfully identified all 10 clinical isolates of Legionella examined in a dot blot assay and should be excellent reagents for use in genuswide diagnostic immunoassays.
Authors:
J S Sampson; B B Plikaytis; C H Aloisio; G M Carlone; C P Pau; A R Stinson
Related Documents :
10463389 - Urinary antigens as markers of papillary toxicity. ii: application of monoclonal antibo...
3701929 - Pathogenicity of antigenic variants of murine coronavirus jhm selected with monoclonal ...
1862939 - Monoclonal antibody to calmodulin: development, characterization, and comparison with p...
22553599 - Bioinformatics-led design of single-chain antibody molecules targeting dna sequences fo...
21864619 - Improving reverse vaccinology with a machine learning approach.
18158789 - A simple method for the production of anti-c3d monoclonal antibody.
1369139 - Influence of ph, nitrogen and phosphorus sources on the production of hepatitis b virus...
1010939 - Antisera to prostaglandins - production and characterization.
11564529 - Measuring of extracellular cortisol and corticotropin-releasing hormone in the amygdala...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  29     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-10-15     Completed Date:  1991-10-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  836-41     Citation Subset:  IM    
Affiliation:
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology*
Antibody Specificity
Bacterial Proteins / immunology*
Hybridomas / immunology*
Legionella / immunology*
Molecular Weight
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Bacterial Proteins
Comments/Corrections
Erratum In:
J Clin Microbiol 1991 Sep;29(9):2092

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Serratia fonticola isolated from a leg abscess.
Next Document:  Inverse correlation in nutritionally variant streptococci between the production of bacteriolytic ac...