Document Detail

Immunologic characterization and specificity of three monoclonal antibodies against the 58-kilodalton protein of Legionella pneumophila.
MedLine Citation:
PMID:  1890189     Owner:  NLM     Status:  MEDLINE    
Three monoclonal antibodies against the Legionella pneumophila 58-kDa protein were produced. By using immunoblot analysis, the percentages of reactivity against 47 serogroups of Legionella representing 29 species were determined to be 80.9, 87.2, and 95.6 for monoclonal antibodies GB5BE8, GB5AF6, and CA4AF5, respectively. Specificities obtained from testing 63 heterologous organisms representing 22 genera and 46 species were 90.7, 92.2, and 95.3% for monoclonal antibodies GB5BE8, GB5AF6, and CA4AF5, respectively. No single heterologous strain was reactive with all three monoclonal antibodies. These monoclonal antibodies successfully identified all 10 clinical isolates of Legionella examined in a dot blot assay and should be excellent reagents for use in genuswide diagnostic immunoassays.
J S Sampson; B B Plikaytis; C H Aloisio; G M Carlone; C P Pau; A R Stinson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  29     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-10-15     Completed Date:  1991-10-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  836-41     Citation Subset:  IM    
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
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MeSH Terms
Antibodies, Monoclonal / immunology*
Antibody Specificity
Bacterial Proteins / immunology*
Hybridomas / immunology*
Legionella / immunology*
Molecular Weight
Sensitivity and Specificity
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Bacterial Proteins
Erratum In:
J Clin Microbiol 1991 Sep;29(9):2092

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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