Document Detail

Immunolocalization of steroidogenic enzymes in equine fetal adrenal glands during mid-late gestation.
MedLine Citation:
PMID:  17585182     Owner:  NLM     Status:  MEDLINE    
To elucidate the relationship between steroidogenic hormones and developing adrenal glands, we investigated the immunolocalization of steroidogenic enzymes in equine fetal adrenal glands during mid-late gestation. Fetal adrenal glands were obtained from three horses at 217, 225 and 235 days of gestation. Steroidogenic enzymes were immunolocalized using polyclonal antisera raised against bovine adrenal cholesterol side-chain cleavage cytochrome P450 (P450scc), human placental 3beta-hydroxysteroid dehydrogenase (3betaHSD), porcine testicular 17alpha-hydroxylase cytochrome P450 (P450c17) and human placental aromatase cytochrome P450 (P450arom). Histologically, cortex and medulla cells were clearly observed in the three fetal adrenal gland tissue samples. P450scc and P450c17 were identified in cortex cells close to medulla cells and in some medulla cells in the fetal adrenal glands. P450arom was present in both cortex and medulla cells in the fetal adrenal glands. However, 3betaHSD was not found in any of the equine fetal adrenal gland tissue samples. These results suggest that equine fetal adrenal glands have the ability to synthesize androgen and estrogen, which may play an important physiological role in the development of equine fetal adrenal glands.
Qiang Weng; Yumiko Tanaka; Hiroyuki Taniyama; Nobuo Tsunoda; Yasuo Nambo; Gen Watanabe; Kazuyoshi Taya
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-22
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  53     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-11-06     Completed Date:  2007-12-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1093-8     Citation Subset:  IM    
College of Biological Science and Technology, Beijing Forestry University, Beijing, P.R. China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
3-Hydroxysteroid Dehydrogenases / analysis
Adrenal Glands / embryology*,  enzymology*
Androgens / biosynthesis
Estrogens / biosynthesis
Fetus / enzymology*
Horses / embryology*
Steroid Hydroxylases / analysis*
Reg. No./Substance:
0/Androgens; 0/Estrogens; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.14.-/Steroid Hydroxylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  High-resolution ultrasonography of xenografted domestic cat ovarian cortex.
Next Document:  Assessment of fertility and reproductive toxicity in adult female mice after long-term exposure to P...