Document Detail


Immunolocalization of steroidogenic enzymes in the corpus luteum and placenta of the Japanese Shiba goat.
MedLine Citation:
PMID:  15699583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we performed immunohistochemistry of cholesterol side-chain cleavage cytochrome P450 (P450scc), 3beta-hydroxysteroid dehydrogenase (3betaHSD), cytochrome 17alpha-hydroxylase P450 (P450c17), and cytochrome P450 aromatase (P450arom) in the corpus luteum and placenta of Shiba goats. The aim was to clarify the steroidogenic capability of the corpus luteum and placenta of Shiba goats. Ovaries containing corpora lutea were obtained from four adult Shiba goats during the luteal phase (day10; n=2) and pregnancy (90 and 120 days of gestation). Placenta was obtained from one Shiba goat on day 120 of gestation. The sections of the ovaries and placentae were immunostained using the avidin-biotin-peroxidase complex method (ABC) with polyclonal antibodies generated against steroidogenic enzymes of mammalian origin. All luteal cells expressed P450scc, 3betaHSD, P450c17 and P450arom. The distribution of P450scc, 3betaHSD, P450c17 and P450arom were not different during the luteal phase and pregnancy. P450arom showed a weak positive staining in late pregnancy (120 days). In addition, immunoreactions for P450c17 and P450arom were observed in syncytiotrophoblast of the placenta of one Shiba goat. These results indicate that, in Shiba goats, corpus luteum is not only an important source of progesterone but also has the ability to synthesize androgen and estrogen during the luteal phase and pregnancy. Also the placenta has the ability to synthesize androgen and estrogen in late pregnancy.
Authors:
Qiang Weng; Mohamed S Medan; LongQuan Ren; Gen Watanabe; Toshio Tsubota; Kazuyoshi Taya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-02-07
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  51     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-10     Completed Date:  2005-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  247-52     Citation Subset:  IM    
Affiliation:
Faculty of Biological Science and Technology, BeiJing Forestry University, China.
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MeSH Terms
Descriptor/Qualifier:
17-Hydroxysteroid Dehydrogenases / metabolism
3-Hydroxysteroid Dehydrogenases / metabolism
Animals
Aromatase / metabolism
Cholesterol Side-Chain Cleavage Enzyme / metabolism
Corpus Luteum / enzymology*
Female
Goats / physiology*
Immunohistochemistry
Luteal Phase / physiology
Placenta / enzymology*
Pregnancy
Progesterone / metabolism
Steroid Hydroxylases / metabolism*
Chemical
Reg. No./Substance:
57-83-0/Progesterone; EC 1.1.-/17-Hydroxysteroid Dehydrogenases; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.1.1.51/3 (or 17)-beta-hydroxysteroid dehydrogenase; EC 1.14.-/Steroid Hydroxylases; EC 1.14.14.1/Aromatase; EC 1.14.15.6/Cholesterol Side-Chain Cleavage Enzyme

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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