Document Detail

Immunolocalization of secretogranin II, chromogranin A, and chromogranin B in differentiating human neuroblastoma cells.
MedLine Citation:
PMID:  1425774     Owner:  NLM     Status:  MEDLINE    
In order to obtain further insights into the expression of the known markers of secretory neuroendocrine dense core organelles, secretogranin II (SgII), chromogranin A (CgA), and chromogranin B (CgB) during neuronal differentiation, the immunolocalization of these proteins was studied by means of double immunofluorescence in both undifferentiated and retinoic acid-differentiated SH-SY5Y human neuroblastoma cells. The majority of undifferentiated cells was not immunolabeled for all three proteins. In the majority of differentiated cells, a clearly punctate SgII immunolabeling indicative of the presence of secretory organelles was present in the Golgi region, at the cell periphery, along the neurites and in growth cones. Only relatively few of the SgII-immunolabeled cells were also immunolabeled for CgA and CgB, and in a single cell the three proteins were not always present in the same organelles. These results, obtained in a cultured cell line, confirm the not necessarily parallel distribution of SgII, CgA, and CgB observed in different neuroendocrine tissues and suggest that SgII may be the best marker of human neuroblastoma cell differentiation.
A M Giudici; E Sher; M Pelagi; F Clementi; A Zanini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cell biology     Volume:  58     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-12-23     Completed Date:  1992-12-23     Revised Date:  2007-04-17    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  383-9     Citation Subset:  IM    
Department of Medical Pharmacology, University of Milano, Italy.
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MeSH Terms
Cell Differentiation
Chromogranin A
Chromogranin B
Chromogranins / biosynthesis*
Golgi Apparatus / secretion
Neurites / secretion
Neuroblastoma / metabolism*
Protein Biosynthesis*
Tretinoin / pharmacology
Tumor Cells, Cultured
Tumor Markers, Biological
Reg. No./Substance:
0/CHGA protein, human; 0/CHGB protein, human; 0/Chromogranin A; 0/Chromogranin B; 0/Chromogranins; 0/Proteins; 0/Tumor Markers, Biological; 302-79-4/Tretinoin

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