| Immunolocalization of retinoic acid biosynthesis systems in selected sites in rat. | |
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MedLine Citation:
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PMID: 15950969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vitamin A deficiency leads to focal metaplasia of numerous epithelial tissues with altered differentiation from columnar (in general) to stratified squamous cells. This process can be reversed with vitamin A repletion. Previously, we described a system of retinoic acid (RA) synthesis in the cycling rat uterus consisting of cellular retinol binding protein (Crbp), epithelial retinol dehydrogenase (eRoldh), retinal dehydrogenase 2 (Aldh1a2), and cellular retinoic acid binding protein type II (Crabp2). Western blot analysis, RT-PCR, and immunohistochemistry were performed to test whether this retinoic acid synthesis system was also present in other vitamin A sensitive tissues. We found that combinations of Crbp, eRoldh, Aldh1a2 or Aldh1a3, and Crabp2 were present in all vitamin A sensitive tissues examined. In the ureter, while eRoldh was present, another short chain alcohol dehydrogenase reductase (possibly Roldh 1, 2, or 3) was in higher concentration in the transitional epithelia. In several tissues, Crbp, Aldh1a2, and/or Aldh1a3 localized to mesenchyme and/or epithelial cells, while eRoldh and Crabp2 were expressed only in epithelial cells. This suggests that mesenchymal-epithelial interactions may be as important in the adult as they are during development and that local synthesis of RA is important in maintenance of these tissues. |
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Authors:
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Helen B Everts; John P Sundberg; David E Ong |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 308 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-01 Completed Date: 2005-09-20 Revised Date: 2008-10-10 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 309-19 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Vanderbilt University Medical Center, Nashville, TN 37232, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Oxidoreductases
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metabolism Aldehyde Oxidoreductases / metabolism Animals Cell Communication / physiology Epithelial Cells / metabolism* Immunohistochemistry Mesoderm / metabolism* Rats Rats, Sprague-Dawley Receptors, Retinoic Acid / metabolism Retinal Dehydrogenase Retinol-Binding Proteins / metabolism* Retinol-Binding Proteins, Cellular Tretinoin / metabolism* Ureter / cytology, metabolism Viscera / cytology, growth & development, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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CA68485/CA/NCI NIH HHS; DK 26657/DK/NIDDK NIH HHS; DK 32642/DK/NIDDK NIH HHS; DK20593/DK/NIDDK NIH HHS; HD 07043/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Rbp1 protein, rat; 0/Receptors, Retinoic Acid; 0/Retinol-Binding Proteins; 0/Retinol-Binding Proteins, Cellular; 0/retinoic acid binding protein II, cellular; 302-79-4/Tretinoin; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.1.105/retinol dehydrogenase; EC 1.14.14.1/Cyp2d1 protein, rat; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.1.36/Retinal Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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