Document Detail

Immunolocalization of regenerating cells after submassive liver necrosis using PCNA staining.
MedLine Citation:
PMID:  1381429     Owner:  NLM     Status:  MEDLINE    
Little data exist on the proliferative state of liver cells and its relationship with various morphological findings in acute liver failure (ALF) in man. In this study we used the monoclonal antibody NCL-PCNA (clone PC-10) against the proliferating cell nuclear antigen (PCNA) to detect cycling cells in paraffin sections of 3 normal livers, 14 post-mortem needle-specimens of submassive hepatic necrosis (SHN) due to paracetamol overdosage (POD), and 10 hepatectomy specimens obtained at transplantation in patients with acute or subacute liver failure of presumed viral aetiology. In normal livers, only occasional sinusoid-lining cells were stained, whereas in SHN following POD or presumed viral hepatitis, hepatocytes of variable morphology showed significant immunoreactivity. Following POD, immunoreactivity was higher in samples taken within 5-6 days than in those obtained at 9-11 days, a pattern reminiscent of the decrease in the rate of regeneration, previously documented after partial hepatectomy in humans. Immunolabelled hepatocytes were aggregated in multiacinar 'nodules' in cases with a map-like distribution of collapsed and non-collapsed parenchyma. Ductules demonstrated comparatively less staining, but extensive labelling was exceptionally found in areas of complete hepatocellular dropout. In these areas, small elongated cells with strongly PCNA-positive ovoid nuclei, forming periportal sprouting cords or incorporated into the lining of ductules, were most remarkable in that they closely resembled 'oval cells' described in animal studies.
G Koukoulis; A Rayner; K C Tan; R Williams; B Portmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of pathology     Volume:  166     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-10-07     Completed Date:  1992-10-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  359-68     Citation Subset:  IM    
Institute of Liver Studies, King's College Hospital, Denmark Hill, London U.K.
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MeSH Terms
Acetaminophen / adverse effects
Acute Disease
Drug-Induced Liver Injury
Immunohistochemistry / methods
Liver / drug effects,  metabolism,  pathology*
Liver Diseases / pathology
Liver Regeneration*
Nuclear Proteins / analysis*
Proliferating Cell Nuclear Antigen
Staining and Labeling
Reg. No./Substance:
0/Nuclear Proteins; 0/Proliferating Cell Nuclear Antigen; 103-90-2/Acetaminophen

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