| Immunolocalization of lymphatic vessels in human fetal knee joint tissues. | |
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MedLine Citation:
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PMID: 20334573 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We immunolocalized lymphatic and vascular blood vessels in 12- and 14-week-old human fetal knee joint tissues using a polyclonal antibody to a lymphatic vascular endothelium specific hyaluronan receptor (LYVE-1) and a monoclonal antibody to podoplanin (mAb D2-40). A number of lymphatic vessels were identified in the stratified connective tissues surrounding the cartilaginous knee joint femoral and tibial rudiments. These tissues also contained small vascular vessels with entrapped red blood cells which were imaged using Nomarsky DIC microscopy. Neither vascular nor lymphatic vessels were present in the knee joint cartilaginous rudiments. The menisci in 12-week-old fetal knees were incompletely demarcated from the adjacent tibial and femoral cartilaginous rudiments which was consistent with the ongoing joint cavitation process at the femoral-tibial junction. At 14 weeks of age the menisci were independent structural entities; they contained a major central blood vessel containing red blood cells and numerous communicating vessels at the base of the menisci but no lymphatic vessels. In contrast to the 12-week-old menisci, the 14-week meniscal rudiments contained abundant CD-31 and CD-34 positive but no lymphatic vessels. Isolated 14-week-old meniscal cells also were stained with the CD-31 and CD 34 antibodies; CD-68 +ve cells, also abundant in the 14-week-old menisci, were detectable to a far lesser degree in the 12-week menisci and were totally absent from the femoral and tibial rudiments. The distribution of lymphatic vessels and tissue macrophages in the fetal joint tissues was consistent with their roles in the clearance of metabolic waste and extracellular matrix breakdown products arising from the rapidly remodelling knee joint tissues. |
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Authors:
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James Melrose; Christopher B Little |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Connective tissue research Volume: 51 ISSN: 1607-8438 ISO Abbreviation: Connect. Tissue Res. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-05 Completed Date: 2011-01-13 Revised Date: 2012-03-02 |
Medline Journal Info:
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Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: England |
Other Details:
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Languages: eng Pagination: 289-305 Citation Subset: IM |
Affiliation:
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Raymond Purves Laboratory, Institute of Bone and Joint Research, Kolling Institute of Medical Research, Royal North Shore Hospital and University of Sydney, St. Leonards, Australia. jmelrose@med.usyd.edu.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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metabolism Antigens, Differentiation, Myelomonocytic / metabolism Blood Vessels / anatomy & histology, metabolism Fetus / anatomy & histology*, blood supply, immunology, metabolism Humans Immunohistochemistry Knee Joint / anatomy & histology*, blood supply, embryology*, immunology Lymphatic Vessels / anatomy & histology*, embryology*, immunology, metabolism Macrophages / cytology, metabolism Membrane Glycoproteins / metabolism Menisci, Tibial / anatomy & histology, blood supply, embryology, metabolism Staining and Labeling Vesicular Transport Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; 0/LYVE1 protein, human; 0/Membrane Glycoproteins; 0/PDPN protein, human; 0/Vesicular Transport Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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