| Immunolocalization of laminin-alpha1-like antigens around synapses in mouse cerebellar perineuronal nets. | |
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MedLine Citation:
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PMID: 14626346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hypothesis that extracellular matrix components may be related to neuronal development in the mouse cerebellar cortex was verified with immunohistochemistry by using an antibody against laminin-alpha1, a major extracellular matrix protein in various tissues. A commercially available polyclonal antibody, raised against the carboxyl-terminal 20-amino acid peptide of laminin-alpha1 was used. Some positive immunoreaction products were localized around large GABAergic interneurons in granular layers and others were around neurons in deep cerebellar nuclei. At the electron microscope level, diaminobenzidine immunoreaction products were localized around presynaptic boutons and in intercellular matrices around interneurons. Such immunoreaction products could be detected at postnatal day 20, when most of cerebellar synapses are assumed to be established. It has been known that a special feature of extracellular matrix, termed perineuronal nets, exists around specific subpopulation of neurons. In the mouse cerebellum, the present findings suggest that laminin itself or laminin-like-antigens exists in the perineuronal nets in relation to inhibitory neuron synapses. |
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Authors:
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Takashi Yagi; Nobuo Terada; Takeshi Baba; Shinichi Ohno |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Histochemical journal Volume: 34 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 2002 |
Date Detail:
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Created Date: 2003-11-19 Completed Date: 2004-06-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: Netherlands |
Other Details:
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Languages: eng Pagination: 559-65 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi 409-3898, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Brain Chemistry Cerebellum / chemistry, metabolism*, ultrastructure Extracellular Matrix / chemistry, metabolism*, ultrastructure Fluorescent Antibody Technique, Indirect Interneurons / chemistry, metabolism*, ultrastructure Laminin / analysis, metabolism* Mice Mice, Inbred C57BL Microscopy, Confocal Microscopy, Immunoelectron Presynaptic Terminals / chemistry, metabolism*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Laminin; 151186-83-3/laminin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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