| Immunolocalization of heparin-binding growth factors (HBGF) types 1 and 2 in rat liver. Selective hyperexpression of HBGF-2 in carbon tetrachloride-induced fibrosis. | |
| | |
MedLine Citation:
|
PMID: 7684779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ito cells play a major role in liver fibrosis but the mechanisms controlling their activation in vivo are poorly understood. Heparin-binding growth factors (HBGF) types 1 and 2 are mitogenic for cultured Ito cells. They have been found in liver extracts but their cellular localization is unknown. We have studied by immunohistochemistry HBGF-1 and -2 expression in normal rat liver and in carbon tetrachloride (CCl4)-induced fibrosis. In normal liver, HBGF-1 was present only in sinusoidal cells whereas HBGF-2 was also detected in endothelial cells lining major vessels. At the acute stage of CCl4 intoxication, HBGF-2 was expressed in centrilobular clusters of mononuclear phagocytes that were surrounded by many HBGF-2-negative Ito cells. In the later stages, HBGF-2 was expressed by Ito cells within the fibrous bands. No modulation of HBGF-1 expression was noted at any stage. These results suggest that (1) at the acute stage of CCl4 intoxication, HBGF-2 produced by mononuclear phagocytes could participate in the recruitment of Ito cells; and (2) during the CCl4-induced fibrotic process, HBGF-2 could contribute to Ito cell proliferation and the synthesis of fibrosis components. In this in vivo model of hepatic fibrosis, the hyperexpression of HBGF-2 is a relatively specific event since the expression of a structurally related molecule, HBGF-1 was not modulated. |
| | |
Authors:
|
F Charlotte; K M Win; A M Preaux; P Mavier; D Dhumeaux; E S Zafrani; J Rosenbaum |
Related Documents
:
|
10519659 - Subcellular distribution of tetrodotoxin in puffer fish liver. 224209 - Hepatocyte suspensions and cultures as tools in experimental carcinogenesis. 6125619 - In vitro measurement of resistance to phalloidin and gamma-glutamyltransferase of carci... 11815289 - Peribiliary myofibroblasts in biliary type liver fibrosis. 20213529 - Cryopreservation of primary porcine liver cells in an organotypical sandwich model in a... 2694719 - Morphological and immunocytochemical examinations on development of b-cells in human em... 2542139 - Role of hydroxyl radicals in escherichia coli killing induced by hydrogen peroxide. 22528219 - Reduced expression of the lrp16 gene in mouse insulinoma (min6) cells exerts multiple e... 18816379 - A novel reverse transduction adenoviral array for the functional analysis of shrna libr... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of pathology Volume: 169 ISSN: 0022-3417 ISO Abbreviation: J. Pathol. Publication Date: 1993 Apr |
Date Detail:
|
Created Date: 1993-06-28 Completed Date: 1993-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0204634 Medline TA: J Pathol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 471-6 Citation Subset: IM |
Affiliation:
|
Unité INSERM 99, Créteil, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carbon Tetrachloride Fibroblast Growth Factor 1 / analysis Fibroblast Growth Factor 2 / analysis Fibroblast Growth Factors / analysis* Immunohistochemistry Liver / chemistry*, pathology Liver Cirrhosis, Experimental / chemically induced, metabolism*, pathology Male Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
|
103107-01-3/Fibroblast Growth Factor 2; 104781-85-3/Fibroblast Growth Factor 1; 56-23-5/Carbon Tetrachloride; 62031-54-3/Fibroblast Growth Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ultimobranchial body nests in human fetal thyroid: an autopsy, histological, and immunohistochemical...
Next Document: Cytokeratin in lens epithelial cells and its effect on anterior lens capsule opacification.