Document Detail

Immunolocalization of heparin-binding growth factors (HBGF) types 1 and 2 in rat liver. Selective hyperexpression of HBGF-2 in carbon tetrachloride-induced fibrosis.
MedLine Citation:
PMID:  7684779     Owner:  NLM     Status:  MEDLINE    
Ito cells play a major role in liver fibrosis but the mechanisms controlling their activation in vivo are poorly understood. Heparin-binding growth factors (HBGF) types 1 and 2 are mitogenic for cultured Ito cells. They have been found in liver extracts but their cellular localization is unknown. We have studied by immunohistochemistry HBGF-1 and -2 expression in normal rat liver and in carbon tetrachloride (CCl4)-induced fibrosis. In normal liver, HBGF-1 was present only in sinusoidal cells whereas HBGF-2 was also detected in endothelial cells lining major vessels. At the acute stage of CCl4 intoxication, HBGF-2 was expressed in centrilobular clusters of mononuclear phagocytes that were surrounded by many HBGF-2-negative Ito cells. In the later stages, HBGF-2 was expressed by Ito cells within the fibrous bands. No modulation of HBGF-1 expression was noted at any stage. These results suggest that (1) at the acute stage of CCl4 intoxication, HBGF-2 produced by mononuclear phagocytes could participate in the recruitment of Ito cells; and (2) during the CCl4-induced fibrotic process, HBGF-2 could contribute to Ito cell proliferation and the synthesis of fibrosis components. In this in vivo model of hepatic fibrosis, the hyperexpression of HBGF-2 is a relatively specific event since the expression of a structurally related molecule, HBGF-1 was not modulated.
F Charlotte; K M Win; A M Preaux; P Mavier; D Dhumeaux; E S Zafrani; J Rosenbaum
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pathology     Volume:  169     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-06-28     Completed Date:  1993-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  471-6     Citation Subset:  IM    
Unité INSERM 99, Créteil, France.
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MeSH Terms
Carbon Tetrachloride
Fibroblast Growth Factor 1 / analysis
Fibroblast Growth Factor 2 / analysis
Fibroblast Growth Factors / analysis*
Liver / chemistry*,  pathology
Liver Cirrhosis, Experimental / chemically induced,  metabolism*,  pathology
Rats, Sprague-Dawley
Reg. No./Substance:
103107-01-3/Fibroblast Growth Factor 2; 104781-85-3/Fibroblast Growth Factor 1; 56-23-5/Carbon Tetrachloride; 62031-54-3/Fibroblast Growth Factors

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